الفهرس | Only 14 pages are availabe for public view |
Abstract The use of plants in the practice of medicine represents one of the biggest human uses of the world. Chenopodium album has antiviral, antifungal, anti-inflammatory, antiallergic, antiseptic band immunomodulating activity. Salix subserrata leaves and flowers have anti-rabies activity. Aqueous extracts of Salix subserrata, did show a significant anti-rabies activity. Sonchus Oleraceus plant has been used for the treatment of fever, stasis and inflammation. Ambrosia martima has antimicrobial, anti-inflammatory and antitumor activity. Egypt has the largest burden of HCV infection in the world, with a 10% prevalence of chronic HCV infection among persons aged 15-59 years. The present study aimed to evaluate the antiviral activity of chloroform, ethyl acetate and butanol extract subfractions of methanol extracts of Chenopodium album, Salix subserrata, Sonchus oleraceus and Ambrosia maritima in antiviral experimental model in vitro; WISH (amniotic epithelial Cell line), HepG2 (Hepatocellular Carcinoma Cell line), Hep2 (Larynx Carcinoma Cell line) and MCF7 (Breast Carcinoma Cell line) and Vesicular Stomatitis Virus. Data obtained in the study showed that: 1-Cytotoxicity study and Lethal dose 50(LD50) determination: Chenopodium album exhibited low cytopathic effect against induced cell lines exhibited complete monolayer cell pattern in cell culture after 24 hr of exposure as compared to control. Furthermore, the cytopatic effect graudullay increased from Salix subserrata to Sonchus oleraceus and Ambrosia maritima which exerts high cytopathic on complete monolayer cell culture of cell lines compared to control. LD50 exhibited by Chenopodium album, Salix subserrata and Sonchus oleraceus was 0.1 of acute dose concentration while Ambrosia maritima showed 0.01 of the acute dose which means that cells exposed to Ambrosia maritima was less susceptible to high doses of that plant extract compared to other studied plants and control. |