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العنوان
Evaluation Study of Antiviral Efficacy of New Active Ingredient in vitro
المؤلف
Radwan, Amna Dahy Mohamed.
هيئة الاعداد
باحث / Amna Dahy Mohamed Radwan
مشرف / Sabah Farouk El-Abd
مشرف / Bahgat Abd Elghafar El-Fiky
مشرف / Bahgat Abd Elghafar El-Fiky
الموضوع
Antiviral Efficacy. Molecular Cell Biology.
تاريخ النشر
2013.
عدد الصفحات
68 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
Molecular Biology
تاريخ الإجازة
1/1/2013
مكان الإجازة
جامعة مدينة السادات - معهد الدراسات والبحوث البيئية - Molecular Cell Biology.
الفهرس
Only 14 pages are availabe for public view

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Abstract

The use of plants in the practice of medicine represents one of the
biggest human uses of the world. Chenopodium album has antiviral,
antifungal, anti-inflammatory, antiallergic, antiseptic band
immunomodulating activity. Salix subserrata leaves and flowers have
anti-rabies activity. Aqueous extracts of Salix subserrata, did show
a significant anti-rabies activity. Sonchus Oleraceus plant has been used
for the treatment of fever, stasis and inflammation. Ambrosia martima has
antimicrobial, anti-inflammatory and antitumor activity. Egypt has the
largest burden of HCV infection in the world, with a 10% prevalence of
chronic HCV infection among persons aged 15-59 years.
The present study aimed to evaluate the antiviral activity of
chloroform, ethyl acetate and butanol extract subfractions of methanol
extracts of Chenopodium album, Salix subserrata, Sonchus
oleraceus and Ambrosia maritima in antiviral experimental model
in vitro; WISH (amniotic epithelial Cell line), HepG2 (Hepatocellular
Carcinoma Cell line), Hep2 (Larynx Carcinoma Cell line) and MCF7
(Breast Carcinoma Cell line) and Vesicular Stomatitis Virus.
Data obtained in the study showed that:
1-Cytotoxicity study and Lethal dose 50(LD50) determination:
Chenopodium album exhibited low cytopathic effect against
induced cell lines exhibited complete monolayer cell pattern in cell
culture after 24 hr of exposure as compared to control. Furthermore, the
cytopatic effect graudullay increased from Salix subserrata to Sonchus oleraceus and Ambrosia maritima which exerts high cytopathic
on complete monolayer cell culture of cell lines compared to control.
LD50 exhibited by Chenopodium album, Salix subserrata and Sonchus
oleraceus was 0.1 of acute dose concentration while Ambrosia
maritima showed 0.01 of the acute dose which means that cells exposed
to Ambrosia maritima was less susceptible to high doses of that plant
extract compared to other studied plants and control.