الفهرس 
Virus purification
Peroxidase isozymeOnly 14 pages are availabe for public view
 |  | from | 95 |  |  |
| | | from | 95 | | |
Abstract
Twenty three tomato accessions were used in this study namelyCastle Rock, Strain B, Edkawi, Jubilee, Money maker, Peto86, Ace,Pritchard, UC, L.hirsutum, L..pimpinellifolium, Diamond, Bonita, Tempo, Regina, AL 2838A , LA 3297, LA 3276, LA 3273, LA 3268, LA 3473,Line 1193 and Relian. The plants were grown in under greenhouse conditions. To test for reactions to ToMV, virus isolates were multiplied in
tobacco (Nicotiana tabacum) and purified. To inoculate plants, infected leaf tissue was ground in 0.1 M phosphate buffer (pH 7) and rubbed onto leaves powdered with carborundum dust. Tomato plants ranged from 10-20 cm tall were selected for inoculation with ToMV virus. Thisis Twenty three tomato accessions were used to study the effect of tomato mosaic virus infection at isozyme and protein.The genes Tm-1, Tm-2 and Tm-22 are known to confer resistance to tomato mosaic virus in tomato (Lycopersicon esculentum) plants. A polymerase chain reaction (PCR) based codominant marker was developed to be linked to these genes (Tm-1, Tm-2 and Tm-22) using sequence characterize amplified region (SCAR) markers. The polymorphic markers co-segregated with susceptibility or resistance, as determined by biological assays for ToMV resistance. This method enables the distinction of homozygous and heterozygous individual plants in segregating populations for Tm-22, and provides a convenient and rapid assay for mosaic virus resistance to be used in tomato breeding programs and hybrid seed production.