الفهرس 
introductionOnly 14 pages are availabe for public view
 |  | from | 140 |  |  |
| | | from | 140 | | |
Abstract
Twenty broiler farms were examined clinically for suspected outbreaks of coccidiosis in some govemorates (Sharkia, Kalyoubya, Monofia and Dakahlia) and trials for isolation and identification of local isolates of cecal E.species were carried out. Identification based on clinical, site of infection, pathognomonic lesion and shape of oocysts. The results revealed that, eleven broiler farms suspected to contain cecal coccidiosis while nine broiler farms showed cecal and intestinal coccidiosis.clinical signs were bloody diarrhea, depression, off food, ruffled feathers and DROP in body weight gain. Pathognomonic lesions were petechial hemorrhages on the serosal surface of ceci, severe distention of cecal tubes with thickened cecal wall and cecal contents ranged from brownish in color to coagulated blood or bloody contents and presence of cecal core in the freshly dead broilers collected from nine broiler farms suffered from cecal coccidiosis only. Site of infection proved to be ceci only relevant to direct wet smear and concentration flotation technique of both cecal and intestinal compartments. Morphological examination revealed that cecal E.oocysts was oval uniform in shape. The above mentioned criteria indicated that the isolated field cecal E. species greatly suspected to be E. tenella. The samples from each farm were subjected to DNA extraction followed by peR using seven primer sets represent the ITS-l gene.