الفهرس 
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Abstract
The present experiment was performed to study the effect of E. stiedae infection on biochemical and immune parameters in rabbits . Also, to investigate the effect of Toltrazuril as anticoccidial . This study applied on 45 New Zealand white rabbits one month old divided into 3 groups, each of 15 rabbits. The 1st group kept as healthy control, the 2nd group infected with 10,000 sporulated oocysts of E. stiedae , while the 3rd group infected with 10,000 sporulated oocysts of E. stiedae then treated on the 7th day post infection with Toltrazuril 1m1/litre in drinking water for 5 successive days . The blood samples obtained 1,2 and 4 weeks PI in gp. 2 and PT in gp. 3 .
Results of gp. 2 revealed that hepatic coccidiosis manifested by rough hair , inappetance , mild diarrhoea and hepatomegaly.
The clinico-biochemical analysis showed very highly significant increase in ALT and AST beside highly significant increase in AP along the experimental period , in addition to hyperbilirubinemia caused by an increase in direct , indirect and total bilirubin levels in all duration.
The proteinogram of infected group showed non significant change in total protein in all periods. There were hypoalbuminemia and hyperglobulinemia allover the durations . There was a significant increase in alpha globulin only in the second week; while beta globulin revealed highly significant increase in the 2nd and 4th weeks. Also, hypergammaglobulinemia was observed in all periods .
Regarding the immunoglobulin results, there was a significant increase in IgM in the first week only . While IgG showed a significant increase in the 2nd and 4th weeks .
Leukogram results revealed leukocytosis in the 1st week then leukopenia in the 4th week. Also, lymphocytosis in the 1st week then lymphopenia and neutropenia in the 2nd and 4th weeks. Monocytosis appeared along the experimental period while eosinophils and basophils were insignificantly changed.
Concerning cellular immunity results , it was clear that E. stiedae infection elicited immunosuppressive effect on lymphocyte transformation rate , phagocytosis percentage and killing percentage in the 4th week.
Treatment of hepatic coccidiosis with Toltrazuril during early stages of infection prevents the appearance of any clinical signs on rabbits except rough hair coat in the 2nd week post treatment.
The clinico-biochemical analysis in the treated group as compared with normal control showed very highly significant increase in ALT and highly significant increase in AST , AP , total and direct bilirubin in the 1st week. The medication effect appeared in the second week where highly significant increase in ALT and significant increase in AST , AP and total bilirubin was found, while direct and indirect bilirubin showed insignificant change. In the 4th week there was only significant increase in ALT.
The proteinogram in infected treated group showed insignificant change in total protein, alpha and beta globulin along the experimental period. While there were hypoalbuminemia, hyperglobulinemia and hypergamma-globulinemia was found in the 1st and 2nd weeks then became insignificant change in the 4th week as compared with normal control .
Immunoglobulin results of infected treated group compared with normal control showed significant increase in IgM in the first week only, while IgG showed significant increase in the 1st and 2nd weeks .
Leukogram results revealed leukocytosis in the 1st week only caused by hymphocytosis and monocytosis , while in the 2nd and 4th weeks there was monocytosis , as compared with normal control.
Concerning with cellular immunity results, there were non significant change in LTR , phogocytosis percentage and killing percentage when compared with normal control.