الفهرس 
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Abstract
This work aimed to study the possible prophylactic and curative effects of colchicine alone or/and in combination with parziquantel on experimentally schistosoma-induced hepatic amyloidosis in Syrian golden hamasters by studying different biochemical parameters in serum and tissue sections of live r and spleen.
These include the following:
1- Histopathological changes, using different techniques:
a)light microscopic examination: different stains were used to assess the morphological changes, these were: haematoxyline and eosin, masons trichrome, periodic acid Schiff(pas) and congo red stais.
b)immunoflourecent examination: type III collagen (col3al) will be localized in hepatic tissue by immunoflourecent technique foe all hamsters use.
2-biochemical examination: including detection of different biochemical parameters in serum samples, they are: albumin (g/ dl), total proteins (g/dl) and cholesterols (mg/ dl). Serum globulin was estimated and albumin/ globulin ratio (a/g)was calculated.
In sera of all positive control hamasters (infected not treated) show significant hypoalbuminaemia, hyperglobulinaemia, lower a/g ratio and hypercholesterolaemia when compared to negative control hamsters, (p 0.0001). histopathological changes in liver and spleen revealed amyloid deposition kupffer cells of liver and in the lymphoid follicles of spleen. Other changes were granuloma formation, bilharzial pigments and inflammatory cells infiltration in the portal tract of liver. None of these biochemical and histopathological changes was seen in the negative control hamasters (not infection).
In this work seventy hamsters were infected with +-200s. haemastobium, they were divided into three groups g2: infected not treated (positive control, pc): g3: infected with early treatment at 9 week post infection: g4: infected with late treatment at 15 week post infection. Anther uninfected group was considering as negative control, nc(g1). group 3 and 4 each contain 30 hamsters, were subdivided into three subgroup each of 10 hamsters differ according to the type of treatment (g3a, 4a) were treated with colchicine only col. group (g3b, 4b) were treated with col + pzq. group (g3c, 4c) were treated with pzq only. Five hamsters from each group were sacrificed at 18 week of infection and the other 5 hamsters at 24 week. Both drugs are given orally, colchicine in a dose of 60ug/kg daily unit the time of sacrifice and pzq as 100mg/kg once which was repeated weekly for 3 consecutive week.
No significant difference were observed between col+ pzq9 group and nc group showing a significant regression in the complication followed the infection by this treatment in all the biochemical parameters measured. Partial regression was observed in col9 group. Complete regression in the complication followed the infection was observed in pzq9 group.
No significant differences were observed between col + pzq15 group and nc group in cholesterol and total protein showing a significant regression in the complications followed the infection by this treatment. No regression was observed in col15 group. Partial regression in the complications followed the infection was observed in pzq15 group.
By comparing group g3a with group g4a, g3b with g4b andg3cwith g4c (same duration of sacrifice, same type of treatment, but different time point of initiation of treatment), statistical analysis showed asignificantly better reponse in group treated early.
Complete parasite eradication was achieved in the group treated with praziquantel alone or in combination to colchicine (group g3b, c and g4b,c) where no adult worms were detected in the mesenteric circulation.
Liver granuloma and bilharzial pigments showed significant reduction in group treated with pzq even it taken alone or in combination with col. This reduction is completely when the drug were given early (9wk) and it is partially when the drug were given late (15 wk). liver also showed significant reduction in amyloid deposits and collagen deposits in groups treated with pzq even it taken alone or in combination with col. This reduction is completely when the drugs were given early (9wk) and it is paratially when the drugs were given late (15 wk). spleen amyloid deposits show the same figure like liver amyloid deposits.
We conclude that colchicine has a significant effect on schistosoma- induced hepatic amyloidosis, especially when it given early of infection and in combination with ant-schistosomal drug praziquuantel.