الفهرس 
Introduction & Aim of The WorkOnly 14 pages are availabe for public view
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Abstract
The aim of this study was to investigate the relationship between
microorganisms isolated from neonates and those isolated from surrounding area ,
common infection sites and pathogens in neonatal intensive care unit in Zagazig
University Hospitals . Also, to evaluate the efficacy of combination of disinfectants
solutions to prevent and eradicate biofilm formed and to suggest a new lock
solution that can prevent attachment of bacteria to rayls, catheters and cannulas.
A total 470 isolates were recovered from the samples. The isolates were
identified as: Staphylococcus aureus(150),coagulase negative staphylococci
(150),Pseudomonas aeruginosa (50), Klebsiella pneumonia(100) and no organisms
were detected in 20 samples.Specimens from skin of neonates revealed that most
commonly isolated organisms were S. aureus (37.5%), CoNS (37.5% , K.
pneumoniae (18.75) and P. aerguinosa (6.25%) . Samples from bottle of milk
feeding , feeding tube and pans of water keeping revealed the presence of S.
aureus , CoNS , P. aerguinosa and K. pneumoniae .
In this study the most commonly isolated organisms from incubators were
CoNS (33.75%, S. aureus (31.25%) , K. pneumonia (16.25%) and P. aerguinosa(
8.75%). The obtained results in this study exihibited that ,the isolates from hands
of health care workers were CoNS (37.5%), S. aureus (31.25%), K.pneumoniae
(21.25%), P. aerguinosa (3.75%). In the present study the enviromental samples
(including walls, doors, tables of medications, sinks and floors of neonatal
intensive care units ) revealed the presence of S. aureus (33.75%) , CoNS(28.75%), P. aerguinosa (18.75%) and K. pneumoniae (18.75%).Samples from disinfectants
solutions revealed the presence of S. aureus , CoNS , P. aerguinosa and K.
pneumoniae. In this study the most frequently isolated organisms from NICU were
S. aureus (31.9%), CoNS (31.9%) , P. aerguinosa(10.6%) and K. pneumonia (
21.28%).