الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
 |  | from | 205 |  |  |
| | | from | 205 | | |
Abstract
Resistance to p-lactam antibiotics is an increasing problem worldwide. The emergence
of multidrug resistant bacteria is mainly associated with P- lactamase production, so
development of new diagnostic and therapeutic methods is crucial to keep pace with
continually evolving pathogens. The current research aims to diagnose and treat the P-
Jactam bacterial resistance by using anti p-lactamase serum. To achieve this goal the
following has been done:
1- E. coli and K. pneumoniae isolates obtained from different sources were resistant to
~-lactams in addition to other drugs by disk diffusion sensitivity test.
2- The CMY p-lactamases from a blacMY E. coli isolate was purified by lysozyme
based method.
3- The CMY p-lactamase extracted from standard isolate in addition to standard CMY
enzyme were subjected to sodium dodecycle sulfate gel electrophoresis (SDS-PAGE)
for their purity assessment and determination of their molecular weights. The protein
content of the purified enzyme was determined by spectrophotometer.
4- Polyc1onal anti-CMY p-lactamase antibodies could be produced in immunized
rabbits.
5- Anti- CMY p-lactamase antibodies had the ability to combine with the extracted P-
lactamase through indirect ELISA technique and detect CMY and many AmpC P-
lactamases in a number of E. coli and K. pneumoniae isolates.
6- Exposure of 10 resistant E. coli and K. pneumoniae isolates to p-lactams and other
drugs under the effect of anti p-lactamase sera through broth rnicrodilution MIC test
and total colony count to determine antisera effect and comparing the MIC value and
colony count before and after addition of antisera.