الفهرس | Only 14 pages are availabe for public view |
Abstract Resistance to p-lactam antibiotics is an increasing problem worldwide. The emergence of multidrug resistant bacteria is mainly associated with P- lactamase production, so development of new diagnostic and therapeutic methods is crucial to keep pace with continually evolving pathogens. The current research aims to diagnose and treat the P- Jactam bacterial resistance by using anti p-lactamase serum. To achieve this goal the following has been done: 1- E. coli and K. pneumoniae isolates obtained from different sources were resistant to ~-lactams in addition to other drugs by disk diffusion sensitivity test. 2- The CMY p-lactamases from a blacMY E. coli isolate was purified by lysozyme based method. 3- The CMY p-lactamase extracted from standard isolate in addition to standard CMY enzyme were subjected to sodium dodecycle sulfate gel electrophoresis (SDS-PAGE) for their purity assessment and determination of their molecular weights. The protein content of the purified enzyme was determined by spectrophotometer. 4- Polyc1onal anti-CMY p-lactamase antibodies could be produced in immunized rabbits. 5- Anti- CMY p-lactamase antibodies had the ability to combine with the extracted P- lactamase through indirect ELISA technique and detect CMY and many AmpC P- lactamases in a number of E. coli and K. pneumoniae isolates. 6- Exposure of 10 resistant E. coli and K. pneumoniae isolates to p-lactams and other drugs under the effect of anti p-lactamase sera through broth rnicrodilution MIC test and total colony count to determine antisera effect and comparing the MIC value and colony count before and after addition of antisera. |