الفهرس 
AbstractOnly 14 pages are availabe for public view
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Abstract
Popularity of herbal remedies is increasing globally and at least one quarter of patients with liver diseases use ethnobotanicals. Plant derived natural products such as flavonoids have received considerable attention in recent years due to their diverse pharmacological properties including antioxidant and hepatoprotective activity. Oleaeuropaea and its products have been recognized as important components of a healthy diet because of their phenolic content. The present study was planned to evaluate the potential hepatoprotective effects of the total crude extract of olive leaves at different concentrations against hepatotoxicity induced by acetaminophen in isolated rat hepatocyte in a hope to use it as a cheap food additive for animals. In addition, the mechanism of the expected protective effect of the olive leaves extract was elucidated through assessment of the exposed hepatocytes performance and cytopathological examination by light and electron microscopes. Liver cell performance was evaluated by determination of viability percent of the isolated hepatocytes, the leakage percent of the cytosolic enzymes (LDH, ALT and AST) from the damaged cells as well as determination of lipid peroxide formation (TBARS level) and content of intracellular glutathione. The predictive protective effect was compared with silymarin which is one of the most known potent hepatoprotective agents. Exposure of isolated rat hepatocytes to acetaminophen (7mM) caused a significant decrease in the viability of isolated rat hepatocytes, and a significant increase in the leakage of intracellular enzymes (LDH, ALT, and AST) into the incubation medium. It caused a marked depletion of GSH and a significant increase in lipid peroxidation. The cytopathological examination revealed morphological alterations of hepatocytes. Pre-incubation of hepatocytes with either olive leaves extract (0.05, 0.1 and 0.2% w/v) or silymarin (0.5 mM) exerted marked protective effect against acetaminophen toxicity which was indicated by increasing the viability of hepatocytes when compared to acetaminophen treated group. They decreased the leakage of intracellular enzymes to the medium, the depletion of GSH and lipid peroxidation. The organelles and cytoplasm structures were widely protected against the effect of acetaminophen. These hepatoprotective effects were probably due to their antioxidant activities. The present results indicate that acetaminophen has a potential cytotoxic effect in rat hepatocytes and olive leaves extract can afford a significant protection against acetaminophen–induced hepatotoxicity. Therefore, this study suggests providing a rational basis for the use of olive leaves against hepatotoxicity. It could provide an effective, cheap and accessible source of additive to conventional treatment for hepatic injuries complicated by xenobiotics in Egypt.