الفهرس | Only 14 pages are availabe for public view |
Abstract Acute respiratory tract infections are generally the main hazard to the poultry rearing industry in Egypt leading to severe economical losses. The present study was aimed to standardize a multiplex PCR (mPCR) assay that simultaneously detects and differentiates the four major avian respiratory viruses (AIV, NDV, IBV and ILTV) in one single reaction, as well as the AIV subtypes (H5 and H9) and IBV serotypes (Mass and 4/91).Theses mPCR assays were applied on 100 field samples to apply a molecular survey of avian respiratory viruses in commercial broiler and layer flocks suffering from respiratory manifestations with relatively high mortality rate in different localities of Kafrelsheikh governorate .The obtained results revealed that these mPCR were successfully able to detect and differentiate single and mixed infections with two or more viruses in a single reaction. Further, the results indicate the high occurrence of mixed infections among these viruses which makes it difficult to differentially diagnose the disease by clinical manifestations. |