الفهرس 
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Abstract
The immunized hemolymph of the third larvalinstar of the flesh fly, Sarcophaga argyrostoma was studied after infection with the gram-negative bacteria,Escherichia coli and the gram-positive bacteria,Staphylococcus aureus, independently. The protein banding pattern of the immunized hemolymph was recorded at different time intervals post-injection. A new protein band,with molecular weight 18.4 KDa, was observed in infected larvae which may function in the immune response. The time of induction of this new protein was 12 h postinjection
with E.coli and 8 h post-injection with
Staphylococcus aureus and it continued to be produced untill 32 h p.i. The differential display technique was used
to screen for mRNAs specifically induced upon bacterial infection of larvae and about 15 differentially displayed transcripts were detected. The immunized hemolymph was
tested for its antibacterial activity using the inhibition zone method. It showed an obvious antibacterial activity against the gram (-) bacteria, Escherichia coli which significantly
increased with time till 32 h p.i. Besides, the immunized hemolymph of E. coli-treated larvae showed antibacterial
activity against the gram (-) bacteria, Pseudomonas aeroginosa. No antibacterial activity was detected against any of the tested gram (+) bacteria.