الفهرس 
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Abstract
The chicken tick Argas persicus is a specific parasite of chicken (permenantly domestic fowls) and distributed throughout the warmer region of the world. In Egypt, it is considered as the most serious
ectoparasite infesting chicken.
The conventional methods of pesticide delivery is the traditional methods for tick control (by dipping or spraying). The researches on non-acaricide chemicals increase the interest in studying tick pheromones with special reference to those used in combination with pesticide. However, from the practical use of pheromone/pesticide point of view, it is obvious that more detailed information concerning the roles of these pheromones in
regulating tick behaviour is needed.
In this study, Argas persicus pheromone was analysed to identify its different components qualitatively and quantitatively using : _
1)high performance thin layer chromatography plates (HPTLC) .
2)high performance liquid chromatography (HPLC) .
Argas persicus pheromone was prepared in the laboratory using commercial standards (SIGMA).
A button like PVC objects (decoys) were manufactured in the laboratory, such decoys were impregnated with pesticide, coated with the laboratory prepared pheromone and used as inanimate objects representing Argas persicus females. The decoys were used in the bioassay to determine Argas persicus attractiveness. Delipidized Argas persicus females were coated with the artificial pheromone and used in the bioassay to estimate the attractiveness of Argas persicus male.
Bioassays showed males tick attraction to the pheromone signals and confused both decoys and delipidized females, attempted to mate with them and died by the pesticide poisoning. Males responses to either decoys or delipidized females coated with artificially prepared pheromone reached 66.7 °A) and 73.3 % respectively.
The use of pheromone and acaricide impregnated decoys is clearly advantageous over the conventional control methods for the following
reasons:
1-Inexpensive and easily manufactured.
2-Preferentially target the chicken tick, making them more specifics than conventional methods.
3-Environmentally sound causing relatively less environmental pollution with toxic chemicals when compared with the conventional methods of
pesticide delivery.
4-Require no water, which is a major advantage in drought-prone areas.
5-Require no specific training for application.
6-Require no special facilities or mechanical apparatus for operation.
7-Have a longe lasting effect up to 3 months.
8-Greatly reduce the need for expensive toxic chemicals.
Electrophoretic and immunological characterization studies ofArgas persicus salivary glands protein were done using SDS-PAGE. Protein profile result revealed few polypeptides.
Immunoblotting on nitrocellulose paper (NCP) was done. The obtained result recognized many immunogenic bands. The most intensity stained one ranged from 69 — 200 Kd. Three immunogenic bands were more prominent than others.