الفهرس 
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Abstract
The present study deals with propagation of chicken anemia virus
(CAV) in primary cell culture (Chicken embryo fibroblast “CEF”) as well
as the continuous cell lines (African green monkey kidney “VERO” and
baby hamster kidney “BHK”) instead of the unavailable (Marek’s disease
cell culture “MDCC”).
Chicken anemia virus “CAV” was propagated serially for ten times
in each of primary chicken embryo fibroblast (CEF); African green
monkey kidney (Vero) and baby hamster kidney (BHK) cell cultures.
The obtained results revealed that:
1-All included cell cultures showed a cytopathic effect (CPE)
characterized by cell detachment and subsequent vacculation of the
infected monolayer’s started by 5th to 7th day post infection (DPI) then
began to appear more early by the successive passage to reach the 2nd DPI
within all cell cultures.
2-The highest virus titers were 7.6log10TCID50 /ml in VERO and
7.5log10TCID50 /ml in CEF while BHK yielded virus titer of
6log10TCID50 /ml.
3- As Vero cell culture is more durable available cell culture and
that one yielding the highest virus titer, it was of choice to study the
growth kinetics of CAV. It was found that the cell associated virus
showed a titer higher than that of the cell free one during the first hours
post cell infection then began to become of lower values. The highest
total virus yield was obtained by 72 hours post cell infection.
4- Direct fluorescent antibody technique (FAT) was carried out on
different infected cell cultures (CEF; Vero and BHK) with CAV in order
to confirm the incidence of the virus that induced the observed CPE using
specific antiserum conjugated with fluorecin isothyocyanat. The
technique revealed the presence of intra nuclear inclusions reflected by
positive FAT as apple green reaction.
5- As additional confirmation for the incidence of CAV in infected
included cell cultures, negative contrast electron microscopy was carried
out on harvested infected cells showing icosahedral particles resemble to
those of CAV.