الفهرس 
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Abstract
The present study was designed to investigate the changes in biochemical, hematological parameters, histopathological and immunohistochemical pictures induced by experimental toxicity by paracetamol, fish oil, which are administrated intragastically and thioacetamide which administrated interaperitoneally.
In this study, 90 albino rats (180-190 gram) were used. Rats were divided into six groups. All groups were reared in separated experimental cages as following: G- Group 1; control group for paracetamol (CP): consists of 15 male rats and received distilled water intragastric for only one day as a single dose. H- Group 2; paracetamol-treated group (P): consists of 15 male rats and received paracetamol intragastric at a dose of 1 gm /kg b. wt. as a single dose. I- Group 3; control group for fish oil (CF): consists of 15 male rats and received distilled water intragastric for 8 weeks. J- Group 4; fish oil-treated group (F): consists of 15 male rats and received fish oil intragastric at a level of 35% of total calories for 8 weeks. K- Group 5; control group for thioacetamide (CT): consists of 15 male rats and received physiological saline by intraproteineal injection for 8 weeks. L- Group 6; thioacetamide-treated group (T): consists of 15 male rats and received thioacetamide (TAA), 200 mg/5 ml saline/Kg. i.p for 8 weeks (3 times / week). The experiment was continued for one week in paracetamol group and eight weeks in fish oil and thioacetamide groups. Collection of blood samples from five rats from each group was performed after 1, 2 and 7 days (paracetamol group) and 4, 6 and 8 weeks (fish oil and thioacetamide groups) of treatment. Samples included whole blood for hematological examination and serum for biochemical examination. Also, tissue specimens for histopathological and immunohistochemical examinations were collected. Hematological examination included red blood cell count, haemoglobin determination, packed cell volume, total and differential leukocytic counts. Biochemical parameters included liver function tests (ALT, AST, ALP, GGT, bilirubin), total proteins, albumin, lipid profile (cholesterol, triglyceride, HDL-cholesterol and LDL- cholesterol). Specimens from liver for histopathological and immunohistochemical examinations.
The following results were observed:
1- Paracetamol-treated group:
There was normocytic hypochromic anemia as indicated by significant decreases in Hb concentration after 1 and 2 days and PCV % after 2 days. Also, there were significant decreases in the MCH and MCHC after 1 and 2 days. Meanwhile, there was a significant decrease in RBCs count after 7 days. Regard to total and differential leukocytic count, there were significant increases in TLC and neutrophils after 1 day and in TLC and lymphocytes after 2 days. On the other hand, neutrophils showed a significant decrease after 7 days. There were significant increases in ALT and AST, and there were significant increase in total bilirubin, direct bilirubin and indirect bilirubin levels after 1 and 7 days of treatment with paracetamol, while total bilirubin only increase after 2 days of treatment. A significant increase in the level of
triglyceride after 1, 2 and 7 days. On the other hand, there were non-significant changes in cholesterol, LDL-C, glucose, albumin and total proteins.