الفهرس | Only 14 pages are availabe for public view |
Abstract In this study, a total of 100 Escherichia coli isolates were collected from various clinical sources from some Hospitals of Mansoura, Egypt, and tested for resistance to eleven antimicrobial agents. Resistance was high for ampicillin (92%), amoxicillin (91%), cefotaxime (53%), cefoperazone (52%), ciprofloxacin (61%) and levofloxacin (52%). However, resistance was low for cefoxitin (4%) followed by tobramycin (8%) and gentamicin (13%). The isolated strains of E. coli were screened for heat-stable enterotoxin encoded genes (STp and estA2) and heat-labile enterotoxin encoded genes (LTA and eltB) as well as astA gene, which encodes enteroaggregative E. coli heat-stable enterotoxin 1 (EAST1) using PCR. We found that 83% of E. coli strains were positive, heat stable enterotoxin genes were found in 53% of total E. coli strains tested; 57 strains (57%) were positive for heat labile enterotoxin genes with eltB was more predominant and astA gene was distributed among 55%. Finally, typing some isolated E. coli which contain enterotoxins by investigation their plasmid profile and restriction fragment length polymorphism (RFLP). |