الفهرس 
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Abstract
This study was performed in Internal Medicine & Infectious and Fish Diseases Dept. Faculty of Veterinary. Medicine, Mansoura University:- This study was applied to explain the effect of vaccines to protect cattle against brucellosis and to prevent spreading of the disease between them. This study was planned on two groups, the first group contained 3382 cattle under investigation from 2 private farms and they classified into; 2802 pregnant for several times, 367 heifer and 223 males, whereas the second group contained 2766 cattle spreading randomly with farmers and classified to; 1970 pregnant cows for several times, 686 heifer and 110 males, the time of investigation of this study was during the period from 2008 - 2011. The detection of abortion rates by using of serological tests on sera sample collected from private farms proved to be 1.16%, 1.19%, 0.92% and 1.27% whereas the abortion rates of cows randomly spreading were 2.29%, 1.4%, 2.6% and 2.8% for years 2008, 2009, 2010 and 2011 respectively. Screening tests (Buffered Acidified Plate Antigen: 1.8%, 2.29%, 3.7 % and 3.8% and (Rose Bengal Plate test) was 1.8%, 2.7%,3.6% and 3,6% for years 2008, 2009, 2010 and 2011 respectively. Confirmatory tests (Tube Agglutination test): the percentage of positive samples were 1.8 %, 2.7 %, 3.4 % and 3.6 % for years 2008, 2009, 2010 and 2011 respectively. Polymerase Chain Reaction (P.C.R) was applied as confirmatory test for Brucella species isolates. Brucella microorganism was isolated from both milk sample and tissue samples (Lymph node) from both private farms and animals randomly distributed with farmers. The abortion rates in private farms due to infection with brucellosis were 1.6%, 1.19%, 0.92% and 1.27% for years 2008, 2009, 2010 and 2011 respectively while the abortion rates in individual animals were 2.29% , 1.4%, 2.6% and 2.8% for years 2008, 2009, 2010 and 2011.