الفهرس | Only 14 pages are availabe for public view |
Abstract Summary Current research aims to: - Study and understand the effect of the magnetic field with intensity very low (3mT) on total body fluids of mice injected with (Ehrlich tumor), as well as depressing the osmotic fragility of the blood; blood viscosity; dielectric properties of hemoglobin tumor growth; the relative weights and the survival time of mice. This work contains five chapters and (141) of scientific references and a summary in English and another in Arabic, and we will be Describe, in brief, the contents of this work Chapter I:- This chapter gives a general introduction to the interactions of electromagnetic radiation with biophysical tissues and the effects caused by it on the tissue. Also reviews the history of research in this field and the most important research, published in this field, it also review gives types of magnetic radiation, and it briefly explains methods of interaction between different kinds of magnetic field with living cells Chapter II:- This chapter reviews the definitions and explains the theoretical basis of the electromagnetic properties of Radiology in general and especially magnetic radiation. This chapter is divided into two sections the first section reviews the physical properties of red blood cells. The second section gives an explanation of the biophysical properties that will be studied in this research. Chapter III:- This chapter provides a discussion of previous studies and explains the techniques of measurement used as a basis for this work. This chapter is divided into three sections. First section reviews the materials used in the preparations for the experiment and the device used in the irradiation. The second section explains injection method and the division of experimental animals to different groups. Third section explains the method used to measure: - blood viscosity; osmotic fragility of red blood cells; permittivity and conductivity of Hemoglobin, tumor growth; the survival time of mice; and the relative weight of mice. Chapter IV:- This chapter includes experimental data where this chapter has three sections. First section displays the results and data obtained in measuring the intensity of the magnetic field used in the irradiation. The second section reviews the analysis graphics and data results that have been obtained from the experiment conducted measurements after slaughtering rats irradiated In this experiment, was to display data and curves osmotic pressure; viscosity and properties dielectric (conductivity and permittivity) and showed statistical analysis on the characteristics of dielectric that changes in osmotic pressure for groups of four given Summary - 61 - changes the fact also that the changes that impurity in viscosity between the four experiments given changes statistically significant (P <0.05) For each of the conductivity and permittivity of hemoglobin rats irradiated and non-irradiated has also given significant change where we found that the group injected tumor and exposed to radiation is top groups in the values of each of the electrical conductivity and permittivity. The third Section presents the analysis of data obtained from the study of the tumor and follow-up stages of growth during the experiment measuring the size and measurement of weight after the season at the end of the experiment and notes through the study of the size and a comparison between the group injected, which was exposed to radiation and the group that was not exposed with injected. tumor size in the irradiated group is smaller compared with the beginning of the experiment, while the other group the size increased of the tumor significantly its size at the beginning of the experiment. And also in line with these experiments values of the relative weight to be calculated at the end of experiment for both groups, we found that the geese relative to irradiated group smaller than the nonirradiated group. As for the survival time this part also graphically depicted shows a comparison between the survival time of mice of the two groups bearing tumor and control group and we found through this drawing that the life of the irradiated mice higher compared with the group injected and non-irradiated. Chapter V:- This chapter provides a discussion of the results obtained from the experiments with a summary of what we learned from these results, the appearance of several peaks in the osmofragility differential curves indicates changes in the packing mechanisms between adjacent molecules forming the cellular membrane. The shift of the main peak and CS towards higher or lower concentrations of NaCl indicates changes in the membrane permeability to water molecules. One may find from the osmofragility differential curves the appearance of several peaks, changes in the values of Cs and WHmax for blood samples from groups 1B ,2B and 3B as compared with control group A, which all indicate changes of the membrane elasticity and permeability Our results concerning the effect of EMF exposure on the erythrocytes viscosity showed increase in viscosity of erythrocytes of mice bearing tumor compared to control. where the value of viscosity of groups was 2.61for control group A, while for experimental group 1B, 2B and 3B, was.2.99, 2.28, and 3.31 respectively. And the increase in conductivity observed in our study reflects the permeability damage of the cell membrane with the subsequent loss of ions, electrolytes and intracellular components. The results indicate a strong dispersion in the β region for all the samples from all groups studied. The dielectric dispersion in the β range (0.1-1 MHz) is mainly due to protein and counter ion molecular relaxation. Moreover the results indicated that the values of the σ for Hb molecule from exposed groups to the magnetic field (3mT) were higher than control. One may conclude that the dose 3 mT may cause more damage. Since RBCs membrane play an essential role in the blood flow rate, changes in its biophysical properties will affect its capability for carrying on its metabolic functions since the RBCs counts were changed after exposure of the animals to the magnetic field. Summary - 62 - It is also clear that the exposure to EMF increase the survival time and inhibits tumor growth of mice bearing Ehrlich tumor. This result confirms the anticancer activity of EMF with intensity of 3mT, previous study on tumor growth inhibition using the same animal model was reported. (129) Mice exposed to EMF (3 mT, 50 Hz) showed tumor volume ratio of 1.93 and inhibition ratio of tumor 75.22 % and increased life span of 101.09% with mean survival time of 35.60 days. While sham exposed mice showed tumor volume ratio of 4.65 and inhibition ratio of tumor after treatment 11.49 % and increased life span of 1.56% with mean survival time of 12.60 days. |