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العنوان
Studies on propagation of some ornamental plants by tissue culture technique /
المؤلف
Ahmed, Ahmed Wahba Sayed.
هيئة الاعداد
باحث / أحمد وهبة سيد أحمد
مشرف / محمود عبدالمنعم خفاجي
مشرف / عبد الله محمد أبوالخير
مشرف / فيصل محمد سعداوي
الموضوع
clorox. Kin. sand. peat. perlite and tap water.
تاريخ النشر
2014.
عدد الصفحات
225 p. ;
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
علوم النبات
تاريخ الإجازة
1/1/2014
مكان الإجازة
جامعة المنصورة - كلية الزراعة - Department of Agric. Botany
الفهرس
Only 14 pages are availabe for public view

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from 225

Abstract

Objectives : aiming to propagate Zantedeschia rehmannii cv. Hot Cherry and Amphilophium paniculatum (L.) kunth through tissue culture technique.
Methods & Results :
The first plant: Zantedeschia rehmannii cv. Hot Cherry:
The best explants of Zantedeschia rehmannii cv. Hot Cherry, were buds excised from peeled tubers or from sprouted ones. Clorox at 40% was used in the sterilization process in order to get the highest significant explant survival%. In the multiplication stage, BA at 4 ppm was used to get the highest number of shoots and leaves. The highest number of roots in the rooting stage was induced when IBA at 3 ppm was applied. During acclimatization stage, a mixture of ”sand+peat+perlite” at equal parts (v/v/v) was used as a potting medium, and plants were watered with MS solution at 25% or 50% to ensure good growth characters.
The second Plant: Amphilophium paniculatum (L.) kunth:
Apart from a single paper concerning the chemical constituents of Amphilophium paniculatum (L.) kunth with a pharmaceutical interest, the current study might be the first investigation on this plant from a horticultural point of view on a worldwide scale.
For sterilization of Amphilophium paniculatum (L.) kunth lateral bud explants, clorox at 20% was used to get the highest explant survival%. In the multiplication stage, either BA or Kin at 4 ppm was used to get the highest number of shoots, and IBA at 3 ppm was used to get the highest number of roots in the rooting stage.
Concusion :
For both plants, Zantedeschia rehmannii cv. Hot Cherry and Amphilophium paniculatum (L.) kunth, tap water could be used in both multiplication and rooting stages in preparing media, as using distilled water has no privilege over tap water. This will help saving a good deal of water, power and expenses.