الفهرس 
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Abstract
Lung cancer kills more patients than any other malignancy in the world . Early stage-disease can be treated with curative intent although the risk for relapse is notoriously high. Unfortunately, the majority of lung cancer patients present at an advanced stage. Despite an initial response to treatment, most of these late stage patients will eventually progress on standerd therapy and die from their disease . Despite the complex of nature of lung cancer its molecular underpinnings are becoming increaseingly clear. The target of much research has been on the discovery of natural and synthetic compounds that can be used in prevention and \ or treatment of lung cancer. Nature products of their plants and combination with potent substances in nanocomposide form that exhibited antitumor activity have been discovered. Aim of the work: The main task of this study is to evaluate the antitumor activity of anovelnanocomposide drug in vitro on various cell line and in vivo on mices bearing and non bearing tumor. Experimental designs: a) prepration of anovelnanocomposide drug Formulation of drug Zn Selenite (A),N-Acetyl cysteine(B) and curcumin (c) were mixed together as follows: (A)= 0.5 g (B)=1.0 g (C) =6.0g 3- The novel formula drug was ball milled for 8 hours in the nanoscale to the corresponding nano drug as nano( selenium,N-acetyl cysteine,curcumin). and we got TEM (Transmission electron microscobe . from the TEM studies represents TEM image of the novel nanocomposide (selenium,N-acetyl cysteine, curcumin) ranging from 6.87-12.7 nm in diameter and has spherical shape. 4-ultrasonication for nanocomposide drug by dissolving it in saline at concentrations of 5g\ litre. b) Acute toxicity study ( Determination of LD 50): For testing the toxicity of anovelnanocomposide drug, 100 female swiss mice albino were divided into ten group each group containing 10 mice, orally injected by various doses starting from 10mg \ kg,b.w, with gradual increase up to 50mg\kg.b/w. the dose killed half of mice we start with it. 2-In vitro studies: Evaluation of Cytotoxicity of Novel Nanocomposide Against Hepatocellular Carcinoma Cells (HepG2 Cell Line),Cervical Carcinoma (Hela Cell Line), Breast Carcinoma Cells (MCF7 Cell Line) . Treatment of three cell lines with anovelnanocomposide at different concentration (0-50 micro gram) showed cytotoxicity with 50% cell vability (IC50) at concentration of 31.5 Mg., 42.8, over 50% by order respectively. d) In vivo studies: This study was carried out on 80 females swiss mice and weighted 20-25 gm. Mice were housed in a seprate metal cages under the same constant environmental and nutritional condition through the period of investigation . d.1- Doses and method of administration of different treatments Benzo[a]pyrene at a dose of 100 mg/kg injected intrapretoneally and the same dose after 20 weeks injected also intrapretoneally. Nanocomposide at dose of 0.02 mg orally by stomach tube three times in aweek for 6 weeks then twice aweek for 6weeks, later one in aweek for the end of expriment.. d.2-Mices were classified into 4 groups each cantain 20 mice as follows: 1.Group (1):control group 20 mice not treated with anything negative control(-ve). 2.Group (2):Nanocomposide group, 20 mice treated only with nanocomposide at a dose of 0.02 mg three times in a week for 6 weeks and twice aweek for 6 weeks and finally one dose until the end of experiment. 3.Group (3):Benzo[a]pyrene group,20 mice injected intrapritoneally of benzo[a]pyrene at dose of 100 mg/kg body weight and the same other dose after 20 weeks. 4.Group (4) : ( Nanocomposide+Benzo[a]pyrene) group 20 mice injected by nanocomposide three times for aweek then injected by benzo[a]pyrene at adose of 100 mg/ kg B.W then complete injection orally with nanocomposide three times for 6 weeks, twice times for 6 weeks, only time for rest of experiment completely 30 weeks.during this period Benzo[a]pyrene also injected for another time after 20 weeks from the beginning of experiment.