الفهرس | Only 14 pages are availabe for public view |
Abstract The work presented in this thesis represents a phytochemical investigation of two Amaryllidaceae plants, namely, Narcissus papyraceus and Narcissus tazetta L. This thesis consists of four parts: Part I HPTLC and GC-MS investigation of some extracts of Narcissus papyraceus and Narcissus tazetta L. Chapter 1: Interspecific and ontogenic variability of the alkaloid metabolism of Narcissus papyraceus and Narcissus tazetta L. This part includes a comparative chromatographic screening of the bulbs, roots, stem discs, scales and flowers of N. papyraceus at different stages of flowering, along with bulbs, roots and aerial parts of N. tazetta L. for the alkaloidal content using High Performance Thin Layer Chromatography (HPTLC) technique and Gas Chromatography - Mass Spectrometry (GC-MS). The results of the study show significant differences in the alkaloid composition, type of biosynthesis, main alkaloid and number of alkaloids observed before flowering, at flowering and after flowering. This reflects the importance of the ontogenetic stage at the time of collection of plant material. i- HPTLC The HPTLC screening of extracts of N. papyraceus and N. tazetta L. revealed the presence of various phytoconstitutents, it also confirmed the presence of alkaloids in all the organs of both plants and during all the stages of flowering of N. papyraceus. A higher degree of alkaloid diversity is observed in N. tazetta L. relative to N. papyraceus. Concerning the total quantity of alkaloids in N. papyraceus, generally bulbs precede the race followed by flowers; scales, roots and finally stem discs with the exception to flowering stem discs that have high alkaloidal content. In contrast to N. papyraceus, the bulbs of N. tazetta L. come in the second place regarding the total amount of alkaloids, while the roots show the maximum alkaloidal content. |