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العنوان
Some histological and ultrastructural studies on the eyelids of mature male camels and goats /
المؤلف
El-Naseery, Nesma Ibraheim Mohammad Ali.
هيئة الاعداد
باحث / Nesma Ibraheim Mohammad Ali El Naseery
مشرف / Ahmed Moustafa M. Balah
مشرف / Waheed A. Abdel-Raheem
مشرف / Ahmed M. El-Baz
الموضوع
Histology and Cytology. Camels - History. Goats - Egypt.
تاريخ النشر
2014.
عدد الصفحات
193 p. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
البيطري
تاريخ الإجازة
1/1/2014
مكان الإجازة
جامعة الزقازيق - كلية الطب البيطرى - Histology and Cytology
الفهرس
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Abstract

In the present study, ten healthy mature males of both camels ”Camelus dromedarius”(aged 5-7 years) and goats ”Capra hircus”(aged 2-3 years) were utilized. The superior, inferior and third eyelids from both orbits were excised rapidly, after slaughter of the animals. The specimen for light microscopy were immediately fixed in 10% buffered neutral formalin and processed for paraffin technique. Sections of 6 μm thickness were obtained. They underwent H & E stain, Silver impregnation technique, Crossmon’s trichrome stain, Ab and PAS techniques. Another minute (about 1 mm³) tissue pieces for transmission electron microscopy were fixed in a buffered GA/FA fixative. Then processed till ultrathin sections were cut and stained with uranyl acetate and lead citrate.
Anatomically, the outer surfaces of both superior and inferior eyelids were covered with a dense coat of numerous ordinary hairs and many dispersed long individual tactile hairs. While, their palpebral margins bear many long eyelashes arranged in rows. The internal surfaces of these eyelids were covered with pale red conjunctiva. Great detectable locational and structural differences were observed in the MGs of both animals. In camels, it was an extrapalpebral well-developed flattened sheet structure. It had two attached ends; one with third eyelid fascia and the other end was embedded in medial commissure. The associated fine hairs were detected on the palpebra tertia surface. While, the goat’s MGs were arranged as a single row along the entire length of both superior and inferior eyelids except at lateral and medial commissures. Its MGs were not associated with hairs. In both animals, the third eyelids were situated in the medial canthus with outer palpebral convex and inner bulbar concave surfaces. An ill-defined nictitating and prominent Harderian glands were wrapped around third eyelid’s base. Histologically, the superior and inferior eyelids had two surfaces (outer thin skin and inner conjunctiva) and three margins (anterior, free and posterior). Their epithelium was changed gradually from epidermal.