الفهرس 
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Abstract
This study was designed to compare between the effect of rice bran oil as natural hypolipidemic agent and atorvastatin as synthetic hypolipidemic agent in induced
hyperlipidemic rats.
Study lasts for 21 days and was carried out on a total of 42 male albino rats 60±10 days old that were divided equally into seven groups (6 rats) per each group as follows:
Group I: control group: kept on basal ration and water ad libitum.
Group II: RBO group: kept on basal ration + RBO dose (2.5ml!kg bwt) and given daily by stomach tube.
Group III: Atorvastatin group: kept on basal ration + Atorvastatin (tablet was ground to fine powder and dissolved in distilled water to reach dose (10mg/kg bwt) and given daily by a stomach tube.
Group IV: Hyperlipidemic or (control +ve) group: kept on basal ration and hyperlipidemia was induced via intraperotineal injection of triton (25 mg of triton was dissolved in 1 ml saline to reach dose 250mg/kg bwt) three times! week for two weeks.
Group V: RBO + triton group: kept on basal ration + RBO at the same previous dose by the same route + triton injection at the same previous dose by the same route for the same period.
Group VI: Atorvastatin +triton group: kept on basal ration + Atorvastatin at the same previous dose by the same route + triton injection at the same previous dose by the same route for the same period.
Group VII: RBO + Atorvastatin+ triton group: kept on basal ration + RBO at half of previous dose by the same route + Atorvastatin at half of previous dose by the same route+ triton injection at the same previous dose by the same route for the same period.
Blood samples were collected from eye canthus of rats of all groups after 21 days and serum was separated for determination of:
• Serum lipid profile (TL-TC-TG-vLDL-c — LDL-c and HDL-c) and calculation of atherogenic indices (CRR- AC-AT).
• Liver functions (Alanine aminotransferase - Aspartate aminotransferase - Gamma glutamyle transferase - Total proteins —Albumin - Globulin - Albumin/Globulin ratio).
• Kidney function (Urea - Uric acid).
• Calcium and inorganic phosphorus levels.
Washed erythrocytes were collected for evaluation of:
• Reduced glutathione.
Liver tissues were collected for evaluation of:
• Liver weight and liver/body weight (%)
• Hepatic lipid profile.
• Lipid peroxide and reduced glutathione.
Heart and liver were taken for:
• Tissue histopathology.
Our results and statistical analysis revealed the following:
1- Injection of triton in rats induced hyperlipidemia. As, it significantly increased serum TL, TC, TG, vLDL- c and LDL-c while, it markedly decreased HDL-c as compared to control group.
2- Treatment with RBO alone or half dosage combination with Atorvastatin showed potent hypolipidemic activity than Atorvastatin monotherapy.
3- Hyperlipidemic rats had high atherogenic indices.
4- Treatment of hyperlipidemic rats with RBO, Atorvastatin and their combination lowered these indices. Moreover, RBO and its half dosage combination with atorvastatin gave more protection than Atorvastatin alone.
5- Liver weight was significantly increased in hyperlipidemic rats. However, the addition of RBO, Atorvastatin and their half dosage combination significantly decreased this increase.
6- Hepatic lipid profile was significantly increased in hyperlipidemic rats while, treatment with RBO, Atorvastatin and their half dosage combination markedly lowered this increase.
7- There were significant increase in lipid-peroxidation and a significant decrease in hepatic reduced glutathione concentration and decrease in the activity of the same enzyme in erythrocytes in hyperlipidemic rats.
8- Treatment of hyperlipidemic rats with RBO, Atorvastatin and their half dosage combination significantly decreased hepatic lipid peroxidation while hepatic concentration of reduced glutathione and GSH concentration in erythrocytes were significantly increase.
9- Hyperlipidemia lipidemia resulted in liver insufficiency as indicated by significant decrease in serum level of TP, Alb, globulin and A/G ratio and marked increase in liver enzyme activities.
10-Treatment with RBO, Atorvastatin and their half dosage combination was able to improve liver functions as serum level of TP, Alb and globulin were increased while liver enzymes activities were decreased.
11- Kidney functions (serum urea and uric acid) were significantly increased in hyperlipidemic rats however, treatment with RBO, Atorvastatin and their half dosage combination lowered serum levels of urea and uric acid.
;reased
12- Treatment of induced hyperlipidemic rats with RBO show no significant change in serum calcium level as well as hyperlipidemic rats treated with Atorvastatin show no change in serum calcium and inorganic phosphorus
‘astatin concentration.
13- Liver tissues of the (control, Rice Bran Oil and Atorvastatin groups) showed
normal histological criteria of the hepatic cords and hepatocytes. The heart also
ination revealed normal histological structures of muscular walls and blood vessels.
n with While, in hyperlipidemic rats liver and heart tissues showed, marked fatty change of the hepatocytes and fatty degeneration of heart muscle.
14- Liver and heart from rats treated with RBO and their half dosage combination Ficantly with Atorvastatin showed milder fatty changes as compared to hyperlipidemic
rats and Atorvastatin monotherapy.