الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
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Abstract
Immunophenotypic analysis of hematologic malignancies arising in or involving the bone marrow (BM) can be performed by flow cytometric technique. Flow cytometric immunophenotyping (FCI) is the preferred method when a bone aspirate is obtainable, because FCI allows for a more comprehensive immunophenotypic profile and enables determination of surface monoclonality.
When the BM cannot be aspirated and only BM core biopsy material is available, immunophenotyping can be performed.
Because of FCI offers more comprehensive evaluation of the immunophenotypic profile of hematologic malignancies involving the BM, a method to evaluate the BM core biopsy material by FCI in patients with failed BM aspiration (dry trap) is highly desirable.
All patients in the study were subjected to the following:
• Full history taking.
• Complete clinical examination.
• Laboratory investigations:
- Complete blood picture.
- Bone marrow trephine biopsy. Part of biopsy was used for paraffin embedding and morphological assessment. The other part was taken on RPMI and used after disaggregation for flow cytometry immunophenotyping.
- Immunophenotyping was performed by Becton Dickinson, FACScalibur flow cytometer equipped with cell Quest software using a primary panel of monoclonal antibodies designed for chronic lymphoproliferative disorders.
The aim of this study was to establish a method for flow cytometric immunophenotyping on samples of bone marrow core trephine biopsy in the diagnosis of cases of chronic lymphoproliferative disorders.
The study was conducted on 30 patients with clinical suspicion of chronic lymphoprolifrative disorder.
Bone marrow examination was performed for all cases.
Bone marrow aspirate examination by morphology and flowcytometry revealed bone marrow infiltration in 4 cases, and no bone marrow infiltration in the remaining 26 cases.
Bone marrow biopsy examination revealed bone marrow infiltration in 16 cases, and no infiltration in the remaining 14 cases.
All BMB specimens were submitted for flowcytometic immunophenotyping.
The distribution of specimens was as follow: 7 cases show infiltration (4 cases with B-NHL, 2 cases with T-NHL and 1case with CLL), and 23 cases show negativity for clonal B and T cells.