الفهرس 
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Abstract
This work was carried out to investigate the occurrence of fascioliasis in human and animals (sheep, cattle and buffaloes) in Sharkia province, Egypt. To achieve this purpose, parasitological examination of faecal samples, and immunological examination of serum samples were respectively used for detection of Fasciola eggs and anti-Fasciola antibodies. Age, sex and seasonal distribution of F. gigantica infection in human and animals were also studied. Moreover, Fasciola WW and E/S antigens were prepared in a trial to evaluate their ability to diagnose fascioliasis using FAST-ELISA and Western blot. 4.Occurrence of fascioliasis in human.- F. gigantica eggs were detected in 9 out of 200 humans’ stool (4.5%) collected from different hospital’ laboratories at Sharkia province. 1.1. Sex distribution of F. gigantica infection in human.- F. gigantica was detected in 7/20 females (5.83%) and in 2 /80 males (2.5%). No statistically significant difference was found between the infection rates of F. gigantica in the examined females and males (P>0.05). 1.2. Age distribution of F. gigantica infection in human.- F. gigantica eggs was detected with the percentages of 9.72, 1.69 and 1.45 in the age groups of (1-15), (16-30) and (30) years old, respectively. There was a significant difference among the prevalence of fascioliasis in different age (P 0.05).