الفهرس 
Development of the liverOnly 14 pages are availabe for public view
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Abstract
The present work aimed to study the developmental changes in
the histological and histochemical structure of the liver in albino rats
under the effect of methotrexate and methotrexate with hydrocortisone
by both light and scanning electron microscope.
Fifty albino rat embryos and 105 of both sex rats at different
ages and weights were used in this study and classified as follows:
1- A control group included ten embryos at the 14th and 20th day and
fifteen rats of both sex at two weeks, one month and adult for
investigation of their livers.
2- An experimental group included forty embryos at the 14th and 20th
days and 90 rats of both sex at two weeks, one month and adult for
studying the effect of methotrexate and methotrexate with
hydrocortisone on the liver of these animals.
Every prenatal and postnatal liver was divided into two parts.
One part was fixed in Karnovsky’s fixative and cut into slices to study
the morphology of the structures seen in the cut surface by using the
scanning electron microscope. The other part was divided into many
parts and put in formal saline, formal calcium and Carnoy’s fixative and
stained by haematoxylin and eosin, Mallory’s trichrome, PAS, Gomori’s
method for alkaline phosphatase and methyl green pyronin stains to
demonstrate the general structure, connective tissue, PAS-positive
material, alkaline phosphatase enzyme and nucleic acids respectively.