الفهرس 
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Abstract
The present investigation was carried out at greenhouse at Genetics Dept., Fac. of Agriculture, Zagazig Univ. for obtaining the plant growth of three promissing mutant lines (M-11-1, M-11-2 and M-15-1) and its mother genotype of Atropa belladonna L. Callus tissue culture and cell suspension experiments were carried out at Tissue culture laboratory, Botany Department, National Research Centre. Chemical criteria and HPLC analysis of tropane alkaloids (Atropine and Hyoscine) were determined in in vivo plants and in vitro (callus and cell suspension) at HPLC unit, Central Service Lab., National Research Centre. There was a highly significant effect of lines was reported on callus induction frequency. There were a markedly increases of callus induction frequency by increasing 2,4-D concentrations until 2.0 mg/l. In contrast, there were a markedly decreases in callus induction frequency by increasing NAA concentrations until 2.0 mg/l. Highly significant differences between two concentrations of 2, 4-D (1.0 and 2.0 mg/l) were recorded on callus fresh weight and callus dry weight. M-11-1 line had higher callus fresh weight than the control and other genotypes at 4th subculture under 2.0 mg/l 2,4–D concentration. While, M-15-1 line possessed highly significant of callus fresh weight (mg) than the other lines under 1.0 mg/l NAA concentration Higher concentrations of total alkaloids under in vivo than in vitro (callus tissues) were recorded. The average of total alkaloids in vivo and in vitro under 2.0 mg/l 2,4 –D were (7.439) and (2.166) respectively. In contrast, HPLC results indicated that the behavior of atropine values in vitro (callus tissues) were higher than in vivo (plant leaves). An important results, Hyoscine value of callus increased than in vivo (plant leaves) under control, about twice and half times. More well as for two lines (M-11-1 and M-15-1), the increase of callus hyoscine values than the leaves about 4 th times for M-11-1 callus comparing with leaves of control. These results confirmed the importance of interaction between new genotypes and 2,4-D concentrations. In contrast, callus that grown on MS media supplemented with NAA had only one compound (Hyoscine) and didn’t have atropine .This wide variation of the effect of NAA than 2,4-D were recorded on the resulting of alkaloid compounds. Regarding cell suspension, there were a markedly increases in the growth of cells (PCV, cell fresh weight and cell density) by increasing Putrescine and Potassium nitrate concentrations until 2.0 mg/l. Using HPLC analysis, two compounds (Atropine and Hyoscine) only were discovered in cell suspension under precursor’s additives (putrescine and potassium nitrate). Important remark, these two compounds were produced in cell suspension only, in contrasing at callus tissues, many compounds were recorded. These two compounds (Atropine and Hyoscine) consider as avery important compounds in alkaloids group. These results indicated that there was large variation between expressions of the genes controlling of alkaloid production. The shape of cell growth (callus or cell suspension) plays an important role in alkaloid production. The cell suspension consider as a very suitable for production of Atropine and Hyoscine, as very important alkaloids. These results confirmed that M-11-1 line consider as a very important genotype for production of alkaloids under callus and cell suspension especially for Atropine and Hyoscine compounds.