الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
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Abstract
• End-stage liver failure is a common occurrence in clinical practice nowadays, with HCV being the most common cause of End-stage liver disease in Egypt. The only available treatment for liver failure is liver transplantation. With the increasing demand for organ donors and long waiting lists the problem of organ shortage has come to the surface.
• Xenotransplantation as a possible source for organ recruitment has always been investigated. Many hurdles including biological, physiological, ethical and infectious hurdles hinder tissue harvesting from animal sources.
• Whole organ decellularization technique has been recently investigated to overcome these hurdles. It allows efficient removal of cellular and nuclear material with the antigenic potential preserving most of the ECM components for 3D architecture together with the vascular network as cues for efficient cellular nutrition and oxygenation. Perfusion-decellularization of whole organs is the most widely applied technique.
• In this study, whole organ decellularization technique was applied on Newzealand white rabbits caudate lobes through cannulation of the portal branch to caudate lobe and perfusion with 500 ml Ringer’s solution, 500 ml 1% Triton-X100, 500 ml DI water, 1000 ml 1% SDS and 500 ml DI water to wash out the detergents.
• Characterization of the decllularized liver matrix was performed and the results were compared to the control specimens from the remaining part of the normal rabbit liver. characterization included histological analysis (both H&E and Mason’s trichrome), Ultrastructural analysis using SEM, Assessment of tissue vasculature using both contrast material and corrosion casting technique. Quantitative analysis of collagen and DNA content.
• Results showed efficient decellularization with very few nuclei observed on histological assessment in some slides and only 1.48% DNA residual on quantitative analysis. Also intact portal vasculature was observed on fluoroscopy and corrosion casting. Honey-comb appearance of the decellularized ECM was observed on histological and ultrastructural analysis. Preservation of 74% of collagen content “the main ECM structural component” was achieved.