الفهرس 
Effect of ovarian status
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Abstract
This study aimed to evaluate the influence of the oocyte collection techniques (dissection, aspiration, slicing, and aspiration plus slicing) on yield and quality of oocytes and effect of medium type with or without hormonal addition on in vitro maturation (IVM) of buffalo oocytes. Oocytes were collected from the ovaries by the four methods to obtain recovery rate and classified into compact (COCs), expanded, denuded, partial denuded and degenerated oocytes. Only COCs were IVM in TCM-199 and Ham-F12 with or without hormonal addition (FSH and estradiol). Results showed that the oocyte recovery rate from ovaries was higher (P<0.05) by aspiration plus slicing (84.67%) and slicing (83.30%) than aspiration (72.68%), while dissection technique showed the lowest (P<0.05) oocyte recovery rate (52.04%). Percentage of cumulus oocyte complexes collected by slicing (63.17%) was higher (P<0.05) than aspiration (51.34 %), aspiration plus slicing (51.24%) and dissection (42.03%). The corresponding percentages of expanded cumulus oocytes were 29.93, 30.31, 27.55 and being 32.68%, respectively significantly difference (P<0.05).