الفهرس 
Cover Page Title in EnglishOnly 14 pages are availabe for public view
 |  | from | 89 |  |  |
| | | from | 89 | | |
Abstract
This work aimed to study the effect of CIDR time application during breeding and non-breeding season and gonadotrophin injection on estradiol and progesterone profile as well as diameter and number of follicles in she camels. The second goal was to describe the effect of GnRH injection on incidence of ovulationThis study was carried at Matrouh Research Station , belonging to the Desert Research Center , Ministry of Agriculture in cooperation with the Department of Theriogenology, Faculty of Veterinary Medicine , Alexandria University. Nine healthy one humped she camels (camelus dromedaries) were used. The animals were 9- 13 years old, with average body weight 450±17.3kg .The` experiment was started during the period of 2013-2014.Experimental design Each female camel was randomly assigned to one of the three treatment groups during breeding season (November-April) (Derar,2003) and non- breeding season (May-OctoberAll animals were synchronized by using a progestin-gonadotropin based hormonal protocol. A controlled internal drug release (CIDR) insert (EAZI-BREED,interAge, Hamilton, New Zealand) impregnated with 1.38 progesterone was inserted vaginally after cleaning and washing the perineum region with water and povidine – iodine based detergent solution , moreover, antibiotics was added during application of CIDR (10 ml tetracycline ) into each animal. At time of CIDR withdrawal each female was injected i.m with 4500 IU PMSG (Folligon, Intervet Crop., and Canada) to stimulate ovarian activity. br>Group 1 animals ( n = 3): CIDR was inserted for 7 days. Group 2 animals (n= 3): CIDR was inserted for 10 daysGroup 3animals (n= 3): CIDR was inserted for 14 daysSuper-OvulatioSelected one she-camel from each group and injected by using GnRH analog (Buserelin, 20ug /animal; Receptal, Intervet, Holland) at day 5 after CIDR removal and injection of 4500 IU PMSG i.m at time of CIDR removal to induce ovulation (Nagy and Juhasz,2012were withdrawn from jugular vein of each female camel into dry vacutainer tubes at days 0,3,5,7,9,11, and 13 after CIDR application for group(1), 0,3,5,7,9,11,13, and 15 after CIDR application for group (2) and 0,3,5,7,9,11,13,15,17and 19 after CIDR application for group(3) (day 0 is the day CIDR application). Blood samples were allowed to clot overnight at 4C. The serum was separated by centrifugation at 3000 rpm for 20 minutes and then stored at -20C until estradiol and progesterone assay. Ovarian follicular dynamics was monitored at day 5 after injection of 4500 IU PMSG by ultrasonographic equipment (Medison , Korea) equipped with 5 to 10 MHz linear transducerThe main results1-There are a significant differences (p<0.05) between progesterone profile (ng/ml) during breeding season and non- breeding season in she-camels before, during and after CIDR application for 7 days (group 1) and injections of PMSG 4500 IU at time of CIDR removal2-There are a significant differences (p<0.05) between estradiol profile (pg/ml) during breeding season and non- breeding season in she-camels before, during and after CIDR application for 7 days (group 1) and injections of PMSG 4500IU at time of CIDR removal3-There are a significant differences (p<0.05) between progesterone profile (ng/ml) during breeding season and non- breeding season in she-camels before, during and after CIDR application for 10 days (group 2) and injections of PMSG 4500IU at time of CIDR removal4-There are no significant differences (p>0.05)between estradiol profile (pg/ml)during breeding season and non- breeding season in she-camels before, during and after CIDR application for 10 days (group 2) and injections of PMSG 4500 IU at time of CIDR removal-There are no significant differences (p>0.05) between progesterone profile (ng/ml) and estradiol profile (pg/ml) during breeding season and non- breeding season in she-camels before, during and after CIDR application for 14 days (group 3) and injections of PMSG 4500 IU at time of CIDR removal6-The means follicular diameter and number after CIDR application for 7 days was highest (1.006±0.04cm and 7±2.08) compared to means follicles diameter and number (0.75±0.22cm, 6±1.5and 0.74±0.13cm ,4±1.5) after CIDR application for10 and 14 days, respectively during breeding season and non-breeding season 7- Ultrasonography examination of she-camel injected by using GnRH analog (Buserelin, 20 ug/animal; Receptal, Intervet, Holland) receptal for super-ovulation showed that there are CLs (2.33±0.3).<ConclusionCIDR application for 7 days and injection of PMSG 4500 IU at day 7 increase the means follicles diameter and number of follicles during breeding season and non-breeding season Further in depth studies on a larger number of animals are needed to disclose the pattern of follicular development and the kinetics of progesterone inshe- camel treated with a CIDROvulation has been induced with 20 ug of the GnRH analogue,