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العنوان
Some immunogenic and antigenic studies on avian influenza virus infection in Egypt /
المؤلف
Helal, Ahmed Maher Abdel-Hamid.
هيئة الاعداد
باحث / أحمد ماهر عبد الحميد هلال
مشرف / منال عفيفي علي عفيفي
مشرف / خالد مدين أحمد
مشرف / سوزان سيد المهدي
الموضوع
avian influenza. Vaccines. Eggs.
تاريخ النشر
2015.
عدد الصفحات
187 p. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
البيطري
تاريخ الإجازة
1/1/2015
مكان الإجازة
جامعة القاهرة - كلية الطب البيطري - Poultry and Rabbit diseases
الفهرس
Only 14 pages are availabe for public view

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Abstract

Seventy three isolates of AIV were collected from 11 Egyptian governorates during the period from 2010 to 2014, from different poultry flocks, The clinical signs were cyanosis of comb and wattle, echymosis on the shanks and feet, facial edema and greenish diarrhea and DROP in egg production .On necropsy, diffuse subcutaneous hemorrhages on the feet and shanks, the lung showed congestion and hemorrhages. Petechiae were noticed throughout on serosal surfaces especially on the peritoneum. The ovaries were hemorrhagic contains yolk from ruptured ova. RT-PCR revealed 43 positive for M gene while 26 positive using H gene primer whereas cleaveage site sequence revealed 17 out 73 positive with percentage 58.9%, 35.6% and 23.2%; respectively. AI strain (A/chicken/Egypt/M7217B/2013(H5N1) represent the most common clusters of AIV was reassorted at National research center and then adapted on MDCK cell line for preparation of Tissue culture (TC) vaccine in parallel with egg adapted one. For assessment of protective: Eighty one 7 day old SPF chicks, were divided into four groups (1-4), 20 each in isolators at CLVEB, were challenged at 28 day old with106 / ml pathogenic AIV (A /ch / Egy / 1709-6 /2008 Eu 717857) via intranasal route. All birds of group 3 (Unvaccinated challenged) died at 3rd day post- challenge, whereas unvaccinated unchallenged birds (group 4) survived without showing clinical signs. The results revealed that Protection % of vaccinated groups either with prepared tissue culture AI vaccine or egg adapted AI one were 50%.