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Abstract Lipoinjection is a promising treatment but has some problems, such as unpredictability and a low rate of graft survival due to partial necrosis. Methods To overcome the problems with lipoinjection were developed. a novel strategy known as cell assisted lipotransfer (CAL). In CAL, autologous adipose derived stem (stromal) cells (ASCs) are used in combination with lipoinjection. Astromal vascular fraction (SVF) containing ASCs is freshly isolated from half of the aspirated fat and recombined with the other half. This process converts relatively ASCs-poor aspirated fat to ASCs-rich fat by manual isolation of ASCs .There are two other methods that are used to supplement aspirated fat with ASCs by automated methods using celusion 800 system . |