الفهرس 
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Abstract
A total of fifteen adult Mongrel dogs were selected in this study. All animals were clinically healthy based on physical examination, urinalysis, complete blood count, and serum biochemistry analysis before the experimental induction of diabetes mellitus. The study was carried out to investigate some hematological and biochemical parameters as well as trials of treatment using antioxidant combination including Vitamin C and Vitamin E compared to that of Insulin treatment. All dogs under experiment were divided into 3 equal groups; the first group included normal healthy dogs and served as control group. The second group injected with alloxan for experimental induction of diabetes mellitus and was treated with insulin. The third group was injected by alloxan for experimental induction of diabetes mellitus and treated with insulin and a combination of vitamin E and vitamin C. Regarding the hematological alterations in the second group, there were non-significant changes in RBCs, PCV and monocyte percentages. On the other hand, there was a significant decrease in hemoglobin and eosinophils levels. In the meantime, the total leukocytic count, lymphocyte and neutrophil percentages revealed a significant elevation. The third group showed significant reduction in the total leukocytic count and neutrophils percentages, while there was a significant increase in hemoglobin and eosinophils levels. On other hand there were non-significant changes in RBCs, PCV and monocyte percentages. Fasting serum glucose level, serum insulin, fasting serum fructosamine level and serum C-peptide levels were significantly elevated in the second group. On other hand the third group showed a clinical improvement as there was a significant decrease in their levels compared with the second group. Lipid profile in the second group revealed an elevation in the cholesterol and triglycerides as well as low density lipoprotein (LDL), while there was a significant reduction in the high density lipoprotein (HDL). In the third group the results showed an improvement in lowering the levels of cholesterol, triglycerides and LDL, while the level of HDL was elevated. The second group showed a significant increase in the liver enzymatic activity, total and direct bilirubin, total protein and albumin levels. On the other hand the third group revealed an improvement in lowering the levels of liver enzymes compared with those of the second group. The second group showed significant elevation in oxidative stress markers with reduction in antioxidant levels. Finally the antioxidants supplementation resulted in a reduction in the levels of oxidative stress markers, as well as an increased level of antioxidants profile. The Histopathological examination of pancreatic tissue specimens collected from all necropsied diabetic dogs after the end of the experiment revealed vacuolation and necrosis of islet of Langerhans beside, necrotic acinar cells with interstitial edema, with massive fibrosis which replaced pancreatic tissue, Meanwhile the liver tissue showed severe hydropic degeneration with dysplasia beside, dilation of sinusoids and swollen kupffer cells. Conclusively, antioxidants are suggested to be a therapeutic aid along with insulin, leading to enhancing their therapeutic effectiveness as well as lowering the disease complications.