الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
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Abstract
The search for new types of actinobacteria is a field of the most importance now a days due to the increased resistance of some pathogenic bacterial strains to many types of antibiotics, that made it necessary to search for new organisms have the ability to produce new antibiotics and increasing the efficiency of these organisms on the production of this antimicrobial. Therefore, the effects of gamma irradiation on increasing of antibiotic production of a new actinobacterial strain were studied in this investigation.>The obtained results can be summarized as follow:Isolate no.2 (Streptomyces microflavus AEFO2-HM775973.1G:302495616) showed the highest antimicrobial activity among the ten tested isolates.•Results revealed that the lethal dose after irradiation was 5 KGy and 3.5 KGy, represents the highest antimicrobial activity.•Several morphological changes in colony morphology were detected and the exposure of Streptomyces microflavus AEFO2-HM775973.1G:302495616 to optimum gamma rays at the dose level of 3.5 KGy stimulate pigmentation, enhanced different carbon sources utilization, enhanced starch hydrolyze, coagulatenation of milk, melanin production, production of cellulase enzymes and caseinase enzymes. All this physiological properties enhanced after exposure to gamma radiation in both generations with the special reference to sodium chloride tolerance which increased from 5 to 8%.•The decrease in DNA amount was detected at both first and second generations after irradiation process and the amplified PCR products for all samples were separated at the same molecular size 264bp.•By using DGGE analysis, the DNA samples separated in two different regions using urea and formamide gel depending on the different sequences of DNA which have a different melting behavior.
•Separation and purification of the n-butanol fraction was performed using TLC and TIC analysis indicates purity then mass analysis and IR was detected.