الفهرس 
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Abstract
This study evaluated the activity of some antimicrobial agents which are common used in clinical field against some pathogenic microbes
isolated from wounded patients. Bacterial and fungal samples were< isolated from fifty wounded patients from the Department of General Surgery in Zagazige University Hospitals.
The bacterial isolates were examined against 13 different antibiotics. Also the fungal isolates were examined against 4 different antifungal agents using disc diffusion method. The MIC and MBC for CEP antibiotic were determined for selected multiresistant bacterial isolates.
The bacterial isolates no 17, 25 and 47were identified as Pseudomonas aeruginosa where the bacterial isolates no 69 and 70 were identified as Staphylococcus aureus.
The Mycological study indicated that most isolates were resistant to ketoconazole and isolates no 7 and 8 had identified as Aspergillus flavus and Aspergillus niger.
Moreover, the antimicrobial activity of some volatile oils against the multiresistant bacterial and fungal species was studied. The volatile oils of basil, lemongrass and lavender showed highest antimicrobial activity against the highly resistant bacterial and fungal species.
The effect of chlorine water on bacterial and fungal species increases with increasing the concentration.
In the same respect, the effect of combination of different percentages of MIC between CEP antibiotic, basil oil and chlorine water against the selected P. aeruginosa 17 showed that, the highest inhibition was achieved by combination of 50% MIC of CEP +50% MIC of chlorine water. Also the synergistic effect of triple treatments (Kt, basiloil and chlorine water) was less than the double treatments (Kt+ basil oil)
or (Kt +chlorine water) against the A. flavus and A. niger.
The plasmid DNA detected from P. aeruginosa before and after treatment with sub MICs of CEP antibiotic, basil oil and chlorine water >was 4.47kbp.
On the other hand, TEM studies showed changes in ultrastructure of P. aeruginosa 17 and A. flavus when treated with combination of antibiotic and chlorine water including cell wall, plasma membrane, cytoplasm and cell organelles.