الفهرس 
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Abstract
The discovery of MicroRNAs circulating in the peripheral blood has opened new directions of research to identify new non-invasive ways of diagnosis of HCC. This study aims to evaluate seum level of MicroRNA885p5 as a biochemical marker for Hepatocellular carcinoma in chronic hepatitis C virus- infected Egyptian patients .
Patients were assigned into:
• group (A): 30patients with HCC who were diagnosed among patients of liver cirrhosis which HFLs detected on U/S examination, patients were diagnosed according to EASL guidelines(arterial hypervascularity and venous with late phase washout). (EASL 2012).
• group (B): This group included 15 patients with HCV related liver cirrhosis (Child A-C); diagnosed by clinical ,laboratory &sonographic evendence of liver cirrhosis.
• group (C): This group included 15 patients with chronic hepatitis C; who were diagnosed by seropositivity for HCV antibodies, HCV RNA by RT-PCR and histopathological evidence of chronic hepatitis .
Analysis of our results showed that seum level of MicroRNA885p5 was able to differentiate between cirrhotic & non-cirrhotic with 88.9 % sensitivity & 80 % specifcity these values were not encountered when levels were assesed between HCC &non-HCC with 36.7 %sensitivity & 80% specifcity.
80
Analysis of our results showed that there was a significant correlation between seum level MicroRNA885p5 & age of studied groups.
Different liver function tests & AFP were correlated with seum MicroRNA885p5, there was no significant correlation between seum MicroRNA885p5 & (AFP , ALT) while there was a good correlation between seum MicroRNA885p5 & synthetic liver functions (Albumin &INR).
Also analysis of our results showed that levels of AFP which were further classified into10-200 ng/dl(cutoff value should be set at 200ng/dL) &>200 ng/dl which it is considered to be gray zone for diagnosis of HCC , there was no significant difference between mean value of different levels of AFP & MicroRNA885-p5 in HCC group.
Disease severity was assessed by Child-Pugh classification & MELD score, there was no significant correlation between seum MicroRNA885p5 & MELD score among HCC&cirrhotic groups(n=45).
While There was a significant increase of seum MicroRNA885p5 with the increase of disease sverity assessed by Child-Pugh classification among HCC & cirrhotic groups.
Concerning to BCLC staging for HCC patients (73.3%)were stage A while stage B-D compromised( 26.7%), serum level of MicroRNA885p5 were significantly higher among HCC patients with stage B-D than those with stage A( p value = 0.046).
81
HFL(s) detected on U/S examination among patients with liver cirrhosis were further assessed by spiral CT abdomen,so there was no significant Correlation between seum level of MicroRNA885p5&(Number-Size) of HFL(s).
So MicroRNA885p5 could be considered a possible marker for significant liver damage rather than HCC marker.
The discovery of MicroRNAs circulating in the peripheral blood has opened new directions of research to identify new non-invasive ways of diagnosis of HCC. This study aims to evaluate seum level of MicroRNA885p5 as a biochemical marker for Hepatocellular carcinoma in chronic hepatitis C virus- infected Egyptian patients .
Patients were assigned into:
• group (A): 30patients with HCC who were diagnosed among patients of liver cirrhosis which HFLs detected on U/S examination, patients were diagnosed according to EASL guidelines(arterial hypervascularity and venous with late phase washout). (EASL 2012).
• group (B): This group included 15 patients with HCV related liver cirrhosis (Child A-C); diagnosed by clinical ,laboratory &sonographic evendence of liver cirrhosis.
• group (C): This group included 15 patients with chronic hepatitis C; who were diagnosed by seropositivity for HCV antibodies, HCV RNA by RT-PCR and histopathological evidence of chronic hepatitis .
Analysis of our results showed that seum level of MicroRNA885p5 was able to differentiate between cirrhotic & non-cirrhotic with 88.9 % sensitivity & 80 % specifcity these values were not encountered when levels were assesed between HCC &non-HCC with 36.7 %sensitivity & 80% specifcity.
80
Analysis of our results showed that there was a significant correlation between seum level MicroRNA885p5 & age of studied groups.
Different liver function tests & AFP were correlated with seum MicroRNA885p5, there was no significant correlation between seum MicroRNA885p5 & (AFP , ALT) while there was a good correlation between seum MicroRNA885p5 & synthetic liver functions (Albumin &INR).
Also analysis of our results showed that levels of AFP which were further classified into10-200 ng/dl(cutoff value should be set at 200ng/dL) &>200 ng/dl which it is considered to be gray zone for diagnosis of HCC , there was no significant difference between mean value of different levels of AFP & MicroRNA885-p5 in HCC group.
Disease severity was assessed by Child-Pugh classification & MELD score, there was no significant correlation between seum MicroRNA885p5 & MELD score among HCC&cirrhotic groups(n=45).
While There was a significant increase of seum MicroRNA885p5 with the increase of disease sverity assessed by Child-Pugh classification among HCC & cirrhotic groups.
Concerning to BCLC staging for HCC patients (73.3%)were stage A while stage B-D compromised( 26.7%), serum level of MicroRNA885p5 were significantly higher among HCC patients with stage B-D than those with stage A( p value = 0.046).
81
HFL(s) detected on U/S examination among patients with liver cirrhosis were further assessed by spiral CT abdomen,so there was no significant Correlation between seum level of MicroRNA885p5&(Number-Size) of HFL(s).
So MicroRNA885p5 could be considered a possible marker for significant liver damage rather than HCC marker.