الفهرس 
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Abstract
A taxonomical study was carried to assess the inter- and intraspecific variation
among 34 OTU’s of two Marrubium species distributed along the western Mediterranean
coastal region of Egypt; M. alysson L. and M. vulgare L. Four approaches were
suggested to discriminate among the studied OTU’s; morphological characters,
molecular markers (seed protein electrophoresis and RAPD analyses), vegetation
characteristics, land use types and soil analysis as well as allelopathy as a biochemical
marker. The constructed dendrograms by using the different types of characters
separately or collectively are congruent in the segregation of M. vulgare.
By means of seed protein electrophoresis, M. vulgare is characterized by one
species-specific band at 24.07 KD. However, M. alysson is acquired by four speciesspecific
at 20, 29.14, 47.55 and 52.13 KD. Within the studied OTU’s of M. alysson, four
distinct samples are recognized; each is characterized by particular total number of bands
and percentage of polymorphism.
Random Amplified Polymorphic DNA Analysis (RAPD) is performed through
six operon primers which produce 221 bands, of which 175 bands are polymorphic. Both
M. vulgare and OTU 31 of M. alysson are segregated together in the dendrogram.
Therefore, the present study recommends that OTU 31 of M. alysson can be allocated at
higher rank than biotype.
The present investigation indicates that there is a strong association between the
three biotypes within M. alysson using different taxonomic markers and both
geographical and edaphic factors which strongly influence the evolution of these
populations. The existence of great environmental changes and high intensities of human.