الفهرس 
acknowledgmentOnly 14 pages are availabe for public view
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Abstract
Stem cell is cell which is not specialized and incomplete division does not resemble any specialized cell. But able to form an adult cell it is divided after several divisions in the appropriate circumstances, and its importance comes from being able to form any kind of specialized cells after grow and develop to the desired cell.
Adipose tissue represents an alternative source of stem cells. Subcutaneous adipose depots are accessible, abundant, and replenishable, thereby providing a potential adult stem cell reservoir for each individual. Many groups working independently have shown that adult stem cells derived from white adipose tissues can differentiate along multiple pathways in vitro, including the adipocyte, chondrocyte, endothelial, epithelial, hematopoietic support, hepatocyte, neuronal, myogenic, and osteoblast lineages
The aim of this research is to isolate the mesenchymal stem cells from human adipose tissue. After that we will make an in vitro characterization of mesenchymal stem cell Moreover we will test the ability of these cells to differentiate into osteogenic and detecting the stages of gene translation and protein formation. The yeast has a long history of use in the biotechnology industry and is still the microorganism of choice for most genes cloning experiment. Recombinant DNA technology can be used to produce both industrially and/or medically important protein. The ability to express Osteocalcine gene in yeast (Kluyveromyces lactis) which high yield protein expression and Easy and fast cell transformation.
1- Adipose Tissue Samples.
Excess adipose tissue (fat) is often available in the form of resected tissue from elective plastic surgery procedures (lipectomy), for example, abdominoplasty or breast reduction. Lipoaspirate from liposuction procedures is also a convenient source of tissue for isolation of hMSCs. The fat tissue in lipoaspirate is already significantly dissociated, so processing is simplified. The tissue have been placed in a sterile specimen container at the time of harvest. Tissue stored at ambient temperature and processed within 8 h.