Search In this Thesis
   Search In this Thesis  
العنوان
Characterization Of Coagulase Negative Staphylococci Isolated from Chicken /
المؤلف
mohammed, Ola Mohammed Magdy Mohammed.
هيئة الاعداد
باحث / علا محمد مجدى محمد محمد
مشرف / جاكين كمال عبد الحليم الجاكى
مشرف / أحمد سميرمحمد
مشرف / سعاد عبد العزيز ناصف
الموضوع
Chickens. Staphylococcus.
تاريخ النشر
2015.
عدد الصفحات
147 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
البيطري
تاريخ الإجازة
1/1/2015
مكان الإجازة
جامعة القاهرة - كلية الطب البيطري - Microbiology
الفهرس
Only 14 pages are availabe for public view

from 168

from 168

Abstract

A total of 176 samples (25 sample of broiler chicken, 25 samples of 1 day old imported chicks, 60 samples of imported frozen chicken, 15 samples of chicken fillet, 15 samples of chicken nuggets, 12 samples of chicken shawarma, 12 samples of chicken luncheon and 12 samples of chicken stock cubes) examined bacteriologically for Coagulase negative staphylococci (CNS), 40 samples were positive for coagulase negative staphylococci with a total prevalence of 22.7 %, while 34.6 % were coagulase positive S.aureus . CNS revealed from 20 % of broiler chickens, 12 % of one day old chicks, 15 % of imported frozen chickens, 33.3 % of chicken fillet , 46.6 % of chicken nuggets ,33.3 % of chicken shawarma , 50 % of chicken luncheon and 8.3% of chicken stock cubes, identification ocuured by 3 methods 1- biochemiclly by catalase test , oxidase test and coagulase test, 2- by PCR using 16S rRNA and clfa primers, 3-Integral system stafilococchi kit was used for identification of 40 isolates of CNS which indicated that 15 isolates were S.xylosus (with the highest percentage 37.5% ) ,7 isolates were S.warneri (17.5%) , 6 isolates were S.saprophyticus (15%) , 3 isolates were S.hominis (7.5%) , 4 isolates were S.gallinarum (10%), 3 isolates were S.simulans (7.5%) and 2 isolates were S.sciuri (5%). CNS strains were tested for antimicrobial sensitivity using Integral system stafilococchi kit and the higher sensitivity was showed to Gentamicin, Levofloxacin and Claritromycin, while higher resistance was showed to Amoxicillin + clavulanic acid. Using PCR technique, all CNS isolates were examined for mecA gene and it was detected in 22.5% of the isolates with the higher incidence in S.xylosus (10%), CNS isolates were examined also for enterotoxin genes sea, seb, sec, sed, see but only seb, sec, see were detected and their percentages were as follow 20% , 12.5%, 15% respectively.
In conclusion,coagulase negative staphylococci may represent a public health hazard.