الفهرس 
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Abstract
The efficacy of transfersomes (flexible liposomes) as a novel technique for topical delivery of the hydrophilic tetra-anionic photodynamic sensitizer aluminum(II1) phthalocyanine tetrasulfonate (AIPcS4) was investigated, on mammalian fibroblasts and on Balb/c mice dorsal skin. AIPcS4 was loaded in transfersomes composed of phosphatidylcholine from soy bean and sodium deoxycholate as edge activator (5:1, 10:1, and 15:1 w/w, ratios), resulting in 110-, 160-, and 200-nm mean size vesicles with encapsulation efficiencies of 16, 25, and 30 %, respectively. The prepared transfersomes were found to be deformable and can pass through pores smaller than their own size. In vitro studies on baby hamster kidney-21 fibroblasts revealed twofold enhancement of the photocytotoxicity of AIPcS4 loaded in transfersomes (Trans-AIPcS4), compared to free AIPcS4 dissolved in culture medium. The photocytotoxicity of Trans-AIPcS4 was less dependent on the incubation time with cells, compared to free AIPcS4. TEM observations showed evidence of cell death by apoptosis. Topical application on the dorsal skin of Balb/c mice revealed that both free AIPcS4 and Trans-AIPcS4 exhibited evident photosensitization towards mice skin, but acquiring different regions of skin. Keywords: Transfersomes - Aluminum phthalocyanine tetrasulfonate - Topical Photodynamic therapy.