الفهرس 
Introduction
Drug delivery and controlled polymerizationOnly 14 pages are availabe for public view
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Abstract
We report the synthesis of a new amphiphilic triblock copolymer composed of a hydrophilic polyethylene glycol (PEG) block, a central polyacrylic acid (PAA) block, and a hydrophobic polymethyl methacrylate (PMMA) block. The obtained results can be summarized as follow:1- We evaluated the ability of poly(ethylene glycol-b-acrylic acid-b-methyl methacrylate)(PEG-b-PAA-b-PMMA)copolymer to self-assemble into micelles that encapsulate Nile red (NR; a hydrophobic fluorescent dye) and CTX in their hydrophobic core.2- We report the synthesis of the acid-labile 2,2’-(propane-2,2-diylbis(oxy)-diethanamine linker and its reaction with the carboxylic acid groups in the central PAA block to cross-link adjacent polymer chains forming shell cross-linked micelles (SCLM).3- We compared the release of the loaded cargo (i.e. NR and CTX) from uncross-linked micelles (NSCLM) and SCLM upon incubation in phosphate-buffered saline (PBS) solution at physiologic (pH = 7.4) and endosomal (pH = 5.0) pH values.4- We investigated the uptake of CTX-loaded SCLM and NSCLM into PC-3 and C4-2B prostate cancer cells and the associated anticancer activity compared to free CTX at different drug concentrations.5-We evaluated micelle’s biocompatibility by measuring the hemolysis of red blood cells (RBCs) 6-Platelets aggregation upon incubation with different micelle concentrations.7- We also measured the adsorption of bovine serum albumin (BSA) as a model serum protein to the surface of SCLM and NSCLM as a function of incubation time 8-Subsequent uptake of opsonized micelles by THP-1 macrophages.