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Abstract Stem cells of umbilical cord blood (UCB) have emerged as a useful alternative source of hematopoietic stem cell transplantation (HSCT). UCB transplants have several strengths including immediate availability, low risk of graft-versus-host disease, and ease of HLA matching requirement. One major limitation for successful outcomes of UCB transplantation is the low volume and low cell dose including TNC count and CD34+ HSC count which are needed for good engraftment results. Several studies described a number of factors that may influence the total volume collected, the quantity of UCB CD34+ cells and TNC. The aim of this study was to characterize and enumerate CD 34+ HSC in UCB and to evaluate the impact of certain maternal and neonatal factors on the quantity and quality of the collected UCB units. The study included 40 umbilical cord blood (UCB) samples obtained by puncture of the umbilical vein of umbilical cords of neonates born in Fayoum University Hospital and collected into blood bags containing CPDA-1 as anticoagulant. |