الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
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Abstract
Oilseed crops are major agricultural commodities that are used primarily for nutritional applications. The fungal infection of oilseeds whether in the field or in store is considered a huge social, healthy and economic problems throughout the world, particularly if these fungi have potential to produce lipolytic enzymes and mycotoxin. The main goal of this study was to isolate, identify lipolytic and mycotoxiginic fungi from some oilseeds further physiological studies on isolated fungi were done. The results indicated that 28 fungal isolates were identified and belonging to 3 classes.Aspergillusflavus and Aspergillusniger were the greatest isolates to produce lipase enzyme while A. flavus has the ability to produce aflatoxin B1. The optimal lipase production by Aspergillusnigar and Aspergillus flavus was in medium contain peptone, 3% glucose, 8% olive oil, 0.1% KH2PO4, 0.052% KCL, 0.52%, MgSO4.7H2O, 3% NaNO3, 0.3% yeast extract and 0.002(W/V) FeSO4.7H2O. pH adjusted 7, 8 for incubation period 5, 7 days at 35°C respectively under shacking state. Lipase enzyme was purified and appeared the highest activity at 35ºC, pH7, 8% olive oil as substrate till 90 min. The Best-fit values of Km for enzyme were 2.6Mm/l and Vmax 115.6.mM. the maximum AFB1 production was obtained at 80 % moisture content in maize, 0.850 aw but in solid medium was under dark at 35 ºC for 14 day. Lipase and aflatoxin B1 production was controlled using some abiotic agents, fungi and some lactic acid bacteria strains.The results revealed acetic acid exhibited inhibitory effect on lipase activity and AFB1 followed by Formic acid, Aniline.Boric acid was the strongest in inhibition of lipaseactivity and AFB1. Bacillus subtilis had the greatest antagonistic activity against A. flavus and A.niger. Whilst four microbial strains inhabited only the growth of A. flavus they were Saccharomyces cerevisiae,Goliacodium sp2.Sterptomyces griseorubenandBacillus spp. On the other hand, mixed culture of Lactic acid bacteria YC28, Mixed culture of Lactic acid bacteria TH4 and Trichoderma viride completely failed to show any antagonistic activity against A. flavus and A. niger