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العنوان
Morphological Classification of Different House Dust Mite Species and Studying Their Impact on the Immune Response in Allergic Patients /
المؤلف
Abou Galalah, Doaa Ibrahem Mostafa.
هيئة الاعداد
باحث / دعاء إبراهيم سلامة أبو جلالة
مشرف / وفاء محمد القرش
مشرف / منى محمد كامل السبكي
مشرف / نانسي محمد حربه
الموضوع
Allergies. Allergic patients.
تاريخ النشر
2016.
عدد الصفحات
202 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
علم الأحياء الدقيقة (الطبية)
الناشر
تاريخ الإجازة
13/5/2016
مكان الإجازة
جامعة المنوفية - كلية الطب - قسم الطفيليات الطبية
الفهرس
Only 14 pages are availabe for public view

from 202

from 202

Abstract

Mites are minuscule arthropods belonging to the Arachnid class. They spread almost in all human houses in humid, warm and temperate climate. Their dimensions are about one quarter of a millimeter. This make them impossible to be perceived by common eyes. They live in the dust of a room, especially in beddings, pillows, mattresses, carpets and upholstered furniture. The HDM have specific environmental requirements for their development. The mites tend to be most numerous in warm homes with high humidity. Optimum conditions for growth and development are around 23oc and 70-80% RH. Dust mites cannot survive well at relative humidities below 50 %. The HDM and their allergenic particles are present within homes year round, but people tend to have fewer symptoms during the summer, perhaps because they spend more time outdoors. The allergenic proteins responsible for causing symptoms are contained within the mites themselves (alive or dead), their shed skins and also in their feces. Routine human activity such as housecleaning, walking or playing on carpeting, or making the bed, causes the tiny fecal particles to become airborne and inhaled these fecal pellets rich in proteolytic enzymes, which are responsible for most of indoor allergy. The aim of the present study was to detect, differentiate and identify of different and most predominant HDM species from dust samples collected from rural and urban areas of Menoufia Governorate. Also,estimation of total IgE and specific IgE against prepared antigens from D. farinae, D. pteronyssinus and T. putrescentia This study was conducted on 42 allergic patients, 20 asthma, 11atopic dermatitis and 11 allergic rhinitis patients in addition to 10 healthy controls. Total and specific serum IgE ELISA test were done to sera of all patients and also for the control. Identification of HDM in dust samples collected from different six areas in Menoufia Governorate including rural and urban areas were performed. 202 dust samples from total 282 dust samples were positive for HDM. spite of the presence of HDM in 71.6% dust samples, the density of mites in dust samples were higher in rural areas (81.7%). The highest density was in Melig (90.2%) village (Rural) and the lowest density was in Quisna and Shebin El-Kom (Urban).