الفهرس 
Colon cancer treatmentOnly 14 pages are availabe for public view
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Abstract
Currently, most drugs intended for cancer therapy are not specific to target cancer cells and therefore may be highly toxic to normal and surrounding tissues. In the recent years there is growing interest in development of natural products as anti-cancer and chemopreventive agents with high efficiency and low toxicity. The aim of this study was to extract saponin from different locally plant sources then evaluate the antioxidant and hemolytic activity of soybean saponin. Also, the antitumor activity in human colon cancer cells was evaluated. Saponin was extracted and determined from six different types of legumes. Soybean saponin was partially purified by silica gel column chromatography and confirmed by FTIR and HPLC techniques. The antioxidant and hemolytic activity of soybean saponin were evaluated. In addition, cytotoxic activity of soybean saponin on human colon cancer cells was evaluated by using MTT assay and the apoptotic effect was assessed by measuring caspase-9 activity, apoptosis by Annexin-V and DNA fragmentation.Moreover, SOD activity and GSH level were measured to evaluate if the apoptotic effect of soybean saponin mediated through oxidative stress. The results showed that the highest saponin content was found in soybean with concentration 94.89 mg/g dry weight. FTIR and HPLC was confirmed the soyabean saponin with concentration 20.5mg/gm dry weight. Moreover, Soybean saponin had antioxidant activity with IC50 value of 3.17mg/ml and it had the ability to hemolyse human blood erythrocyte with HD50 value of .412mg/ml. In addition, the incubation of colon cancer cells with different concentrations of soybean saponin for 48hr decreased the viability of cells in dose dependent manner and increased the activity of caspase-9 enzyme compared to the untreated cells. Flow cytometric analysis was carried out on the treated cells showed that soybean saponin had apoptotic effect. In addition, soybean saponin increased the levels of reduced glutathione and the activity of superoxide dismutase when measured in the lysate of the treated cells. In conclusion, soybean saponin had antioxidant and hemolytic activity. In addition, it had antitumor activity against human colon cancer cell line and its mechanism is mediated through apoptotic effect.Keywords:Saponin, Soyabean, Colon cancer, antitumor activity, apoptotic effect.