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العنوان
Detection of Staphylococcus Aureus Classic Enterotoxin Genes in some Meat Products Using Multiplex PCR /
المؤلف
Ibrahim, Shimaa Mohamed Abd El-Bar.
هيئة الاعداد
باحث / شيماء محمد عبد البرإبراهيم
مشرف / نادر يحيى مصطفى
مشرف / عزة مرغنى محمد ديب
مشرف / سهام نزيه حموده
الموضوع
Food contamination.
تاريخ النشر
2016.
عدد الصفحات
187 p. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
البيطري
تاريخ الإجازة
13/3/2016
مكان الإجازة
جامعة كفر الشيخ - كلية الطب البيطري - قسم مراقبة الأغذية
الفهرس
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Abstract

A total of 200 samples of sausage (50), beef burger (50), minced meat (40) and luncheon (60) were randomly collected from different shops and markets in El-Gharbia and Kafr-El-Sheikh governorates. The samples were subjected to bacteriological examination and molecular characterization. The results revealed that the all meat product samples especially luncheon were highly contaminated with staphylococci and S. aureus, also the use of multiplex PCR with specific primers for S. aureus classic enterotoxin genes revealed that only one isolate for sausage sample mostly harboured (1) sea, meanwhile one isolate for beef burger sample harboured (1) seb gene and the other one was negative for classic enterotoxin genes, but the four isolates of minced meat samples harboured (1) sea, (1) sec, (1) mixed sea & sed genes and one was negative for classic enterotoxin genes and the samples were negative o SEE.