الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
 |  | from | 212 |  |  |
| | | from | 212 | | |
Abstract
Immobilized enzymes bound to polymeric supports are prepared for the purpose of repeated use and the possibilities of continuous reaction systems. One of the most important properties is the stability of proteins when they are used in some medical and industrial applications. Immobilization of enzymes improves this property as well as many other properties. In the present study three enzymes were immobilized, namely ADA Adenosine Deaminase [EC
3.5.4.4, Type IV; from Bovine Spleen], Pullulanase [EC 3.2.1.4.1, from Klebsiella pneumoniae] and 13-Amylase [EC 3.2.1.2., Type 11-B from Barley). The immobilization of these enzymes was carried out onto three different polymeric materials: agarose, casein and butylacrylate/ acrylic acid (BuA/ AAc) copolymer. The factors affecting the amount of enzyme attachment onto the polymeric supports such as incubation pH, enzyme concentration and incubation time was investigated. Some physico-chemical properties of the free and immobilized enzymes such as reuse efficiency, optimum temperature and thermal stability, pH optimum and stability, storage stability and the effect of y-radiation were studied. For the immobilization process, chemical and radiation methods were used. The results of this study may be summarized as follows:
• The maximum amount of enzyme immobilized onto the different polymeric supports occurred at incubation pH values of 7.5, 5 and 7 for Adenosine Deaminase (cone. 42 units/ gm), Pullulanase (cone. 16 units/gm) and J3-amylase (86 units/ gm), respectively. The incubation time needed for the three enzymes was found to be
8, 10 and 11 hours, respectively. An optimum concentration of the enzymes and optimum incubation time were determined....