الفهرس 
INTRODUCTION
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Abstract
The present studies were carriedout in insectory at Plant Protection Institute, ARC, Giza to aim enhancement methods of efficacy of the entomopathogenic nematodes to killing their hosts and promising alternative to chemical insecticides for the control of a wide variety of economically important insect pests.
Entomopathognic nematodes, the heterohabditis and steinernemtids are lethal insect parasites used in the biological control of insects.
Successful parasitization by these nematodes depends mainly upon four processes: 1) Find the host; 2) Penetration the host; 3) Killing the host and 4) reproducing inside the host and producing new progeny. Enhancing one or more of the first three processes will lessen the exposure of these juveniles to unfavorable environmental conditions, thus better control result will be achieved which is the aim of the present investigation, through carrying out three enhancement methods of efficacy of the entomopathogenic nematodes Sterinemema riobrave and Sterinemema rarun and Heterorhabditis sp and Heterorhabditis .indica (ISK-2 strain)
5.1. In the first method, continuous culture of S.riobrave and S.rarun and H.sp and H. indica juveniles, for several cycles under optimum condition of temperature (25°C) and nematode density (20IJs/ larva), inside thehost (full grown larvae of the greater wax moth, Galleria mellonella ) was carried out.
The mortality and the penetration rate and the virulence (one: one assay) tested in S.riobrave and S.rarum and H.sp and H.indica after 1st, 2nd, 3rd, 4th, 5th and 6th cycles. The new juveniles and original nematode progenies of the four species, were then impairedas regards the mortality, penetration rate, virulence.