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Abstract Diabetes mellitus is one of metabolic disorders characterized by hyperglycemia resulting from defects in insulin secretion, insulin action, or both. The chronic hyperglycemia of diabetesis associated with dysfunction, and failure of variousorgans. Diabetic nephropathy (DN), is a serious microangiopathic complication of diabetes mellitus, and it is leading to end stage renal disease. The development of DN is highly influenced by genetic factors and hyperglycemia. Methylenetetrahydrofolate reductase (MTHFR) is an enzyme important for Homocysteine metabolism. Severe MTHFR deficiency is associated whith hyperhomocysteinemia. The MTHFR C677T gene polymorphism causes an alanine to valine amino acid substitution, this results in a thermo labile MTHFR enzyme with reduced catalytic activity.This mutation may be associated with hyperhomocysteinemia. This mutation and hyperhomocysteinemia were the concern of many studies to asses if they were associated with DN in type 2 diabetes mellitus patients. This study was conducted on 50 persons, 20 control normal persons and 30 type 2 diabetes mellitus patients. The MTHFR C677T gene mutation was detected by PCR-RFLP. The results showed that the presence of MTHFR C677T polymorphism (CT+TT) is not significantly related to increased risk of development of macroalbuminuria nor or microalbuminuria. There is no association of polymorphism and macroalbuminuria. The presence of 677T allele also not significantly associated to increased risk of developing macroalbuminuria. |