الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
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Abstract
Abstract
Meat and meat by products were taken from various areas in Egypt (Cairo, Sharqia, Ismailia and Suez). The samples were subjected to bacteriological isolation of salmonella spp. Fifteen samples gave a suspected growth on the selective agar media. After biochemical isolation they were identified as salmonella spp
The total 15 isolates were sampled from Cairo (4 positive samples), Mansoura (2 positive samples), Ismailia (2 positive samples), Sharqia (2 positive samples) Fayoum (2 positive samples) and finally Suez governorate (3 positive samples). Two of these samples were identified as S. typhi (isolated from Suez governorate) and one identified as S. paratyphi (isolated from Fayoum governorate). All the samples were further identified by serotyping and the results were all S. typhimurium except the two strains which were identified by biochemical reaction as S. typhi and S. paratyphi that gave the same results by serotyping.
The isolated strains were further identified by traditional real time PCR or the portable identification system (R.A.P.I.D). All samples gave a positive Ct.
Whole genome sequencing using ion torrent PGM The developed contigs were assembled and 4.1 mega bases have been developed when single nucleotide polymorphism analysis was conducted, about 9401 SNPS have been found.