الفهرس 
IntroductionOnly 14 pages are availabe for public view
 |  | from | 87 |  |  |
| | | from | 87 | | |
Abstract
Diabetic foot infection represents a major concern for diabetic foot patients as it is increasing rapidly with more virulent organisms. These virulent organisms include the flesh and bone eating bacteria which produce proteolytic enzymes in order to hydrolyze the skin, flesh and bone. When infection spreads, physicians lean to amputate the infected foot then follow the procedure with long term treatment with broad spectrum antibiotics. Even after complete eradication of bacteria, their proteolytic enzymes remain in the blood circulation which leads to prior amputation.
Isolation of 33 bacterial samples was done from 10 diabetic foot ulcers patients. After biochemical identification it was found that they were 24 Gram negative and only 9 Gram positive. The most distributed were Proteus mirabilis and Pseudomonas aeruginosa. These bacterial samples were inoculated onto different protein sources to evaluate which has the highest proteolytic activity. P. mirabilis, P. aeruginosa, S. pyogenes and S. aureus were found to have the ability to hydrolyze hyaluronic acid which is one of the main constituents of human‟s connective tissue.